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In vitro establishment and characterization of two acquired
immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2)
containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA
sequences
E Cesarman, PS Moore, PH Rao, G Inghirami, DM Knowles and Y Chang
Department of Pathology, New York Hospital-Cornell Medical Center, New York
10021, USA.
Two unique DNA fragments were recently identified in over 90% of acquired
immunodeficiency syndrome (AIDS)-related Kaposi's sarcoma tissues. Sequence
analysis suggests that these fragments belong to a previously unidentified
human herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV). These
fragments have also been identified in a subset of non-Hodgkin's lymphomas
in human immunodeficiency virus- positive patients; specifically, in body
cavity-based lymphomas (AIDS- BCBLs). We have established two cell lines
derived from AIDS-BCBLs, BC- 1 and BC-2, which retain the KSHV sequences,
and have used them to further characterize this putative viral genome. In
this report, we demonstrate that the KSHV sequences represent a portion of
a much larger DNA molecule that is located predominantly in the nucleus of
the infected cells. In situ hybridization of metaphase spreads made from
both of these cell lines show these sequences in episomal structures. Their
presence in the cells as large nuclear episomes supports previous sequence
homology data suggesting that KSHV belongs to the herpesvirus family. These
cell lines provide a continuous source of KSHV sequences. Thus, they
represent an important tool for future studies of this recently described
human herpesvirus that may be involved in the pathogenesis of Kaposi's
sarcoma and some AIDS-related non-Hodgkin's lymphomas.
Volume 86,
Issue 7,
pp. 2708-2714,
10/01/1995
Copyright © 1995 by The American Society of Hematology
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C. Lim, C. Choi, and J. Choe
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