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Blood, Vol. 114, Issue 19, 3983-3993, November 5, 2009
Modeling the functional heterogeneity of leukemia stem cells: role of STAT5 in leukemia stem cell self-renewal
Blood Heuser et al.
114: 3983
Supplemental materials for: Heuser et al
Files in this Data Supplement:
- Table S1. Six-day in vitro LSC expansion potential of MN1+CTL and MN1+ND13 cells§ (PDF, 14.6 KB)
- Table S2. Six-day in vitro LSC expansion potential of wildtype MN1+HOXA9 and Stat5b−∕− MN1+HOXA9 cells§ (PDF, 65.5 KB)
- Table S3. Six-day in vitro LSC expansion potential of wildtype MN1+HOXA9 and Stat1−∕− MN1+HOXA9 cells§ (PDF, 65.2 KB)
- Table S4. STAT gene sets from Molecular Signatures Database (PDF, 14.2 KB)
- Table S5. Interleukin gene sets from Molecular Signatures Database (PDF, 12 KB)
- Table S6. Primer sequences (PDF, 60.7 KB)
- Figure S1. STAT and MAPK signalling in MN1+HOXA9 transduced cells in the two-oncogene compared to the one-oncogene leukemia models (JPG, 145 KB)
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(A, B) Cytokine stimulation assay of murine bone marrow cells stably transduced with MN1+HOXA9 or MN1+CTL. Cells were cultured in the presence of the indicated cytokines (6 ng/mL IL-3, 10 n/mL IL-6, 20 ng/mL SCF, or 10 ng/mL GM-CSF), and viable cells were counted and replated every 3 days (mean ± SD, n=3).
- Figure S2. Jak2 inhibition does not have a differential effect on one- compared to two-oncogene immortalized-bone marrow cells (JPG, 19 KB)
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MN1+CTL or MN1+ND13 cells were incubated at a cell density of 1 × 104 cells/mL in the presence of either ethanol (1/1000th of total volume, = control) or increasing concentrations of tyrphostin AG 490. After 72 hours viable cells were counted. Proliferation inhibition is expressed as percent growth of control (mean ± SD of 2 independent experiments).
- Figure S3. Cytokine expression in the two-oncogene compared to the one-oncogene leukemia models (JPG, 167 KB)
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Quantitative gene expression analysis of potential autocrine or paracrine cytokines in leukemic cell lines MN1+CTL and MN1+ND13 in relation to gene expression in the non-leukemic cell line ND13 (mean ± SD, n=3). $ND13 was used as a non-leukemic calibrator.
- Figure S4. HOX cluster gene expression in the two-oncogene compared to the one-oncogene leukemia models (JPG, 64.7 KB)
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Quantitative gene expression analysis of HoxA and HoxB cluster genes in leukemic cell lines MN1+CTL and MN1+ND13 in relation to gene expression in the non-leukemic cell line ND13 (mean ± SD, n=2).
- Figure S5. Interleukin/Jak signalling related gene sets are depleted in AML compared to normal bone marrow or peripheral blood cells (JPG, 39.5 KB)
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Results of gene set enrichment analysis with 5 Interleukin/JAK signalling gene sets in AML patient subgroups using previously published gene expression profiles. Bars represent number of significantly depleted gene sets in subgroup (scale on left axis), line represents mean normalized enrichment score of the 5 gene sets tested (scale on right axis). There are no significant differences for comparison of NES scores in patients with complex karyotype/−5/−7 with any other cytogenetic or molecular subgroup except for AML with t(15;17), P=.006.
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