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Blood, Vol. 112, Issue 3, 493-503, August 1, 2008
Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation
Blood Bonecchi et al.
112: 493
Supplemental materials for: Bonecchi et al
Files in this Data Supplement:
- Video 1. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 2.78 MB)
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CHO-K1/D6 cells untreated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green; EEA1) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy. For all videos, high-resolution (800 × 800 pixels) z-stack was obtained from a representative field from one experiment of three independently performed. Depth is indicated in the lower left corner. Images were compressed with QuickTime Pro software.
- Video 2. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 2.8 MB)
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CHO-K1/D6 cells treated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green; EEA1; Rab4) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy.
- Video 3. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 1.67 MB)
-
CHO-K1/D6 cells untreated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green; Rab4) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy.
- Video 4. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 1.74 MB)
-
CHO-K1/D6 cells treated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy.
- Video 5. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 3.06 MB)
-
CHO-K1/D6 cells untreated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green; Rab11) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy.
- Video 6. Z-stack analysis of D6 colocalization with endosomal markers (MOV, 1.07 MB)
-
CHO-K1/D6 cells treated with CCL3L1 (100nM, 30 min) were stained for D6 (red), endosomal marker (green; Rab11) and with DAPI for nuclear labeling (blue). Cells were then examined by confocal microscopy.
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