|
|
 Previous Article | Table of Contents | Next Article 
PU.1 (Spi-1) and C/EBP alpha regulate the granulocyte colony- stimulating
factor receptor promoter in myeloid cells
LT Smith, S Hohaus, DA Gonzalez, SE Dziennis and DG Tenen
Department of Medicine, Beth Israel Hospital, Harvard Medical School,
Boston, MA 02215, USA.
Cytokines, important for lineage commitment and differentiation during
hematopoiesis, exert their influence by binding specific receptors.
Receptor expression is tightly regulated and examining the factors that
govern their expression will allow better understanding of the events that
determine lineage commitment. The granulocyte colony-stimulating factor
(G-CSF) receptor is expressed exclusively in myeloid cells and the
placenta. We show here that the G-CSF receptor transcription start site is
identical in each of these tissues. A 1,391-bp fragment of the G-CSF
receptor promoter is both active in myeloid cell lines and tissue specific.
We have also found two regions that are important for G-CSF receptor
promoter activity. One region, located at bp -49, contains a GCAAT site
that specifically binds the C/EBP alpha transcription factor in myeloid
nuclear extracts. Mutation of this site prevents C/EBP alpha binding and
reduces promoter activity by 60%. The other functionally important region
of the G-CSF receptor promoter is in the 5' untranslated region, at bp +36
and +43, where there are two sites for the ets family member PU.1. Mutation
of these sites prevents PU.1 binding and reduces promoter activity by 75%.
These results reinforce the importance of both PU.1 and C/EBP alpha in the
expression of myeloid-specific genes and neutrophil development.
Volume 88,
Issue 4,
pp. 1234-1247,
08/15/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Ji, H. Li, H. C. Suh, K. D. Klarmann, Y. Yokota, and J. R. Keller
Id2 intrinsically regulates lymphoid and erythroid development via interaction with different target proteins
Blood,
August 15, 2008;
112(4):
1068 - 1077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Iida, R. Watanabe-Fukunaga, S. Nagata, and R. Fukunaga
Essential role of C/EBPalpha in G-CSF-induced transcriptional activation and chromatin modification of myeloid-specific genes.
Genes Cells,
April 1, 2008;
13(4):
313 - 327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-J. Zhang, L.-Y. Ma, Q.-H. Huang, G. Li, B.-W. Gu, X.-D. Gao, J.-Y. Shi, Y.-Y. Wang, L. Gao, X. Cai, et al.
Gain-of-function mutation of GATA-2 in acute myeloid transformation of chronic myeloid leukemia
PNAS,
February 12, 2008;
105(6):
2076 - 2081.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Chang, R. Santhanam, R. Trotta, P. Neviani, A. M. Eiring, E. Briercheck, M. Ronchetti, D. C. Roy, B. Calabretta, M. A. Caligiuri, et al.
High levels of the BCR/ABL oncoprotein are required for the MAPK-hnRNP-E2 dependent suppression of C/EBP{alpha}-driven myeloid differentiation
Blood,
August 1, 2007;
110(3):
994 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, J. Grewal, and H. P. Koeffler
ATF4 differentially regulates transcriptional activation of myeloid-specific genes by C/EBP{epsilon} and C/EBP{alpha}
J. Leukoc. Biol.,
June 1, 2007;
81(6):
1535 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. C. Suh, J. Gooya, K. Renn, A. D. Friedman, P. F. Johnson, and J. R. Keller
C/EBP{alpha} determines hematopoietic cell fate in multipotential progenitor cells by inhibiting erythroid differentiation and inducing myeloid differentiation
Blood,
June 1, 2006;
107(11):
4308 - 4316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. U. Mueller, T. Pabst, J. Fos, V. Petkovic, M. F. Fey, N. Asou, U. Buergi, and D. G. Tenen
ATRA resolves the differentiation block in t(15;17) acute myeloid leukemia by restoring PU.1 expression
Blood,
April 15, 2006;
107(8):
3330 - 3338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. T. Porse, T. A. Pedersen, M. S. Hasemann, M. B. Schuster, P. Kirstetter, T. Luedde, I. Damgaard, E. Kurz, C. K. Schjerling, and C. Nerlov
The Proline-Histidine-Rich CDK2/CDK4 Interaction Region of C/EBP{alpha} Is Dispensable for C/EBP{alpha}-Mediated Growth Regulation In Vivo
Mol. Cell. Biol.,
February 1, 2006;
26(3):
1028 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, U. Krug, J. O'Kelly, E. An, V. Vegesna, and H. P. Koeffler
Aberrant expression of neutrophil and macrophage-related genes in a murine model for human neutrophil-specific granule deficiency
J. Leukoc. Biol.,
November 1, 2005;
78(5):
1153 - 1165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Paz-Priel, D. H. Cai, D. Wang, J. Kowalski, A. Blackford, H. Liu, C. A. Heckman, A. F. Gombart, H. P. Koeffler, L. M. Boxer, et al.
CCAAT/Enhancer Binding Protein {alpha} (C/EBP{alpha}) and C/EBP{alpha} Myeloid Oncoproteins Induce Bcl-2 via Interaction of Their Basic Regions with Nuclear Factor-{kappa}B p50
Mol. Cancer Res.,
October 1, 2005;
3(10):
585 - 596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shimokawa and C. Ra
C/EBP{alpha} functionally and physically interacts with GABP to activate the human myeloid IgA Fc receptor (Fc{alpha}R, CD89) gene promoter
Blood,
October 1, 2005;
106(7):
2534 - 2542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Helbling, B. U. Mueller, N. A. Timchenko, J. Schardt, M. Eyer, D. R. Betts, M. Jotterand, S. Meyer-Monard, M. F. Fey, and T. Pabst
CBFB-SMMHC is correlated with increased calreticulin expression and suppresses the granulocytic differentiation factor CEBPA in AML with inv(16)
Blood,
August 15, 2005;
106(4):
1369 - 1375.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. F. Johnson
Molecular stop signs: regulation of cell-cycle arrest by C/EBP transcription factors
J. Cell Sci.,
June 15, 2005;
118(12):
2545 - 2555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okuno, G. Huang, F. Rosenbauer, E. K. Evans, H. S. Radomska, H. Iwasaki, K. Akashi, F. Moreau-Gachelin, Y. Li, P. Zhang, et al.
Potential Autoregulation of Transcription Factor PU.1 by an Upstream Regulatory Element
Mol. Cell. Biol.,
April 1, 2005;
25(7):
2832 - 2845.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Helbling, B. U. Mueller, N. A. Timchenko, A. Hagemeijer, M. Jotterand, S. Meyer-Monard, A. Lister, J. D. Rowley, B. Huegli, M. F. Fey, et al.
The leukemic fusion gene AML1-MDS1-EVI1 suppresses CEBPA in acute myeloid leukemia by activation of Calreticulin
PNAS,
September 7, 2004;
101(36):
13312 - 13317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Halmos, D. S. Basseres, S. Monti, F. D'Alo, T. Dayaram, K. Ferenczi, B. J. Wouters, C. S. Huettner, T. R. Golub, and D. G. Tenen
A Transcriptional Profiling Study of CCAAT/Enhancer Binding Protein Targets Identifies Hepatocyte Nuclear Factor 3{beta} as a Novel Tumor Suppressor in Lung Cancer
Cancer Res.,
June 15, 2004;
64(12):
4137 - 4147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rosenbauer, K. Wagner, P. Zhang, K.-P. Knobeloch, A. Iwama, and D. G. Tenen
pDP4, a novel glycoprotein secreted by mature granulocytes, is regulated by transcription factor PU.1
Blood,
June 1, 2004;
103(11):
4294 - 4301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. K. Hwang, C. S. Kim, H. S. Choi, S. R. McKercher, and H. H. Loh
Transcriptional Regulation of Mouse {micro} Opioid Receptor Gene by PU.1
J. Biol. Chem.,
May 7, 2004;
279(19):
19764 - 19774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schwieger, J. Lohler, M. Fischer, U. Herwig, D. G. Tenen, and C. Stocking
A dominant-negative mutant of C/EBP{alpha}, associated with acute myeloid leukemias, inhibits differentiation of myeloid and erythroid progenitors of man but not mouse
Blood,
April 1, 2004;
103(7):
2744 - 2752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Maun, P. Gaines, A. Khanna-Gupta, T. Zibello, L. Enriquez, L. Goldberg, and N. Berliner
G-CSF signaling can differentiate promyelocytes expressing a defective retinoic acid receptor: evidence for divergent pathways regulating neutrophil differentiation
Blood,
March 1, 2004;
103(5):
1693 - 1701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. V. Hedvat, J. Yao, R. A. Sokolic, and S. D. Nimer
Myeloid ELF1-like Factor Is a Potent Activator of Interleukin-8 Expression in Hematopoietic Cells
J. Biol. Chem.,
February 20, 2004;
279(8):
6395 - 6400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Frohling, R. F. Schlenk, I. Stolze, J. Bihlmayr, A. Benner, S. Kreitmeier, K. Tobis, H. Dohner, and K. Dohner
CEBPA Mutations in Younger Adults With Acute Myeloid Leukemia and Normal Cytogenetics: Prognostic Relevance and Analysis of Cooperating Mutations
J. Clin. Oncol.,
February 15, 2004;
22(4):
624 - 633.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Ross, H. S. Radomska, B. Wu, P. Zhang, J. N. Winnay, L. Bajnok, W. S. Wright, F. Schaufele, D. G. Tenen, and O. A. MacDougald
Phosphorylation of C/EBP{alpha} Inhibits Granulopoiesis
Mol. Cell. Biol.,
January 15, 2004;
24(2):
675 - 686.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tavor, D. J. Park, S. Gery, P. T. Vuong, A. F. Gombart, and H. P. Koeffler
Restoration of C/EBP{alpha} Expression in a BCR-ABL+ Cell Line Induces Terminal Granulocytic Differentiation
J. Biol. Chem.,
December 26, 2003;
278(52):
52651 - 52659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. D'Alo', L. M. Johansen, E. A. Nelson, H. S. Radomska, E. K. Evans, P. Zhang, C. Nerlov, and D. G. Tenen
The amino terminal and E2F interaction domains are critical for C/EBP{alpha}-mediated induction of granulopoietic development of hematopoietic cells
Blood,
November 1, 2003;
102(9):
3163 - 3171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhu, D. M. Giannola, Y. Zhang, A. J. Rivera, and S. G. Emerson
NF-Y cooperates with USF1/2 to induce the hematopoietic expression of HOXB4
Blood,
October 1, 2003;
102(7):
2420 - 2427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Keeshan, G. Santilli, F. Corradini, D. Perrotti, and B. Calabretta
Transcription activation function of C/EBP{alpha} is required for induction of granulocytic differentiation
Blood,
August 15, 2003;
102(4):
1267 - 1275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Throm and M. J. Klemsz
PU.1 regulates glutathione peroxidase expression in neutrophils
J. Leukoc. Biol.,
July 1, 2003;
74(1):
111 - 117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kim, Y. Ogata, and R. A. Feldman
Fes Tyrosine Kinase Promotes Survival and Terminal Granulocyte Differentiation of Factor-dependent Myeloid Progenitors (32D) and Activates Lineage-specific Transcription Factors
J. Biol. Chem.,
April 18, 2003;
278(17):
14978 - 14984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, S. H. Kwok, K. L. Anderson, Y. Yamaguchi, B. E. Torbett, and H. P. Koeffler
Regulation of neutrophil and eosinophil secondary granule gene expression by transcription factors C/EBPepsilon and PU.1
Blood,
April 15, 2003;
101(8):
3265 - 3273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-T. H. Truong, Y.-J. Lee, T. A. Lodie, D. J. Park, D. Perrotti, N. Watanabe, H. P. Koeffler, H. Nakajima, D. G. Tenen, and S. C. Kogan
CCAAT/Enhancer binding proteins repress the leukemic phenotype of acute myeloid leukemia
Blood,
February 1, 2003;
101(3):
1141 - 1148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Schuster, K. Forster, H. Dierks, A. Elsasser, G. Behre, N. Simon, S. Danhauser-Riedl, M. Hallek, and M. Warmuth
The effects of Bcr-Abl on C/EBP transcription-factor regulation and neutrophilic differentiation are reversed by the Abl kinase inhibitor imatinib mesylate
Blood,
January 15, 2003;
101(2):
655 - 663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Vangala, M. S. Heiss-Neumann, J. S. Rangatia, S. M. Singh, C. Schoch, D. G. Tenen, W. Hiddemann, and G. Behre
The myeloid master regulator transcription factor PU.1 is inactivated by AML1-ETO in t(8;21) myeloid leukemia
Blood,
January 1, 2003;
101(1):
270 - 277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Okuno, C. S. Huettner, H. S. Radomska, V. Petkova, H. Iwasaki, K. Akashi, and D. G. Tenen
Distal elements are critical for human CD34 expression in vivo
Blood,
December 15, 2002;
100(13):
4420 - 4426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Du, M. J. Stankiewicz, Y. Liu, Q. Xi, J. E. Schmitz, J. A. Lekstrom-Himes, and S. J. Ackerman
Novel Combinatorial Interactions of GATA-1, PU.1, and C/EBPepsilon Isoforms Regulate Transcription of the Gene Encoding Eosinophil Granule Major Basic Protein
J. Biol. Chem.,
November 1, 2002;
277(45):
43481 - 43494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. O'Donnell, L. J. Druhan, and B. R. Avalos
Molecular characterization and expression analysis of leucine-rich {alpha}2-glycoprotein, a novel marker of granulocytic differentiation
J. Leukoc. Biol.,
September 1, 2002;
72(3):
478 - 485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. U. Mueller, T. Pabst, M. Osato, N. Asou, L. M. Johansen, M. D. Minden, G. Behre, W. Hiddemann, Y. Ito, and D. G. Tenen
Heterozygous PU.1 mutations are associated with acute myeloid leukemia
Blood,
July 18, 2002;
100(3):
998 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Behre, S. M. Singh, H. Liu, L. T. Bortolin, M. Christopeit, H. S. Radomska, J. Rangatia, W. Hiddemann, A. D. Friedman, and D. G. Tenen
Ras Signaling Enhances the Activity of C/EBPalpha to Induce Granulocytic Differentiation by Phosphorylation of Serine 248
J. Biol. Chem.,
July 12, 2002;
277(29):
26293 - 26299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Henkel, S. R. McKercher, and R. A. Maki
Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells
Int. Immunol.,
July 1, 2002;
14(7):
723 - 732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. A. Reddy, A. Iwama, G. Iotzova, M. Schulz, A. Elsasser, R. K. Vangala, D. G. Tenen, W. Hiddemann, and G. Behre
Granulocyte inducer C/EBPalpha inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions
Blood,
June 28, 2002;
100(2):
483 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhang, E. Nelson, H. S. Radomska, J. Iwasaki-Arai, K. Akashi, A. D. Friedman, and D. G. Tenen
Induction of granulocytic differentiation by 2 pathways
Blood,
May 29, 2002;
99(12):
4406 - 4412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q.-f. Wang and A. D. Friedman
CCAAT/enhancer-binding proteins are required for granulopoiesis independent of their induction of the granulocyte colony-stimulating factor receptor
Blood,
April | |
