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Effects of recombinant human megakaryocyte growth and development factor on
platelet activation
G Montrucchio, MF Brizzi, G Calosso, S Marengo, L Pegoraro and G Camussi
Dipartimento di Fisiopatologia Clinica, Universita di Torino, Italy.
The c-Mpl receptor for thrombopoietin and its recombinant related protein,
the megakaryocyte growth and development factor (MGDF), is also expressed
on circulating platelets. In the present study we evaluated the effect of
MGDF on platelet aggregation in platelet-rich plasma (PRP) and in whole
blood. The results obtained indicate that MGDF by itself did not affect
platelet aggregation. However, when added before other agonists such as
adenosine diphosphates (ADP), epinephrine (EPI), and thrombin (THR), it
rendered platelets more sensitive. This "priming" effect of MGDF was
dependent on the dose and on the time of platelet preincubation, and it
occurred both in PRP and in whole-blood platelet aggregation. MGDF also
"primed" the release of adenosine triphosphates and the production of
thromboxane B2 by platelets stimulated with ADP, EPI, and THR. When added
15 seconds after the preincubation of platelets with subthreshold
concentrations of ADP, EPI, and THR, MGDF exhibited a synergism with these
agonists. Moreover, we observed a "priming" effect of MGDF on the
phosphorylation of p-42 mitogen-activated protein kinase promoted by ADP,
EPI, and THR. These observations suggest that thrombopoietin may play a
physiologic role in modulating the response of platelets to several stimuli
and thereby their hemostatic potential.
Volume 87,
Issue 7,
pp. 2762-2768,
04/01/1996
Copyright © 1996 by The American Society of Hematology
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