|
|
 Previous Article | Table of Contents | Next Article 
1,25-dihydroxyvitamin D3 differentiates normal neutrophilic promyelocytes
to monocytes/macrophages in vitro
K Nakamura, T Takahashi, Y Sasaki, R Tsuyuoka, Y Okuno, M Kurino, K Ohmori, S Iho and K Nakao
College of Medical Technology, Kyoto University, Japan.
Although it is well established that the addition of 1,25- dihydroxyvitamin
D3 (D3) to the culture of normal human granulocyte/macrophage progenitors
induces monocyte/macrophage (Mo/M phi) colonies, the target cells of D3 in
the Mo/M phi differentiation have not been identified. We examined whether
neutrophilic promyelocytes are the target cells. As a source of the
promyelocyte fraction, we used colonies after 5 days of culture (5-day
colonies) of colony-forming unit-granulocyte. The culture contained
granulocyte colony-stimulating factor (G-CSF) as the growth factor and
generated only neutrophilic colonies. The promyelocytic nature of the 5-day
colonies was confirmed morphologically, cytochemically, and
ultrastructurally. After morphological evaluation on part of the individual
colonies, they were transferred into new semisolid cultures with or without
D3 (10(-7) mol/L) in the presence of G-CSF, then incubated for the
subsequent 7 days. With D3, the colonies were loose, and all the
constituent cells were morphologically small macrophages, which were
positive for alpha-naphthyl butyrate (alpha NB) esterase, strongly positive
for CD14 antigen, and plastic-adherent. While without D3, the colonies were
rather compact, and all the constituent cells were morphologically mature
neutrophils, which were positive for naphthol ASD-chloroacetate esterase
and weakly positive for CD14 antigen. Secondary culture of the 8- or 10-day
colonies with D3 induced a lower number of alpha NB-positive cells, in
proportion to the percentage of promyelocytes at the time of transfer in
each colony. Four days of secondary culture with D3 was sufficient to
induce alpha NB-positive cells. G-CSF was not an essential factor to induce
alpha NB- positive cells. These findings indicate that D3 differentiates
normal human neutrophilic promyelocytes into the Mo/M phi lineage in vitro.
Volume 87,
Issue 7,
pp. 2693-2701,
04/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Karlsson, G. Carlsson, O. Larne, M. Andersson, and K. Putsep
Vitamin D3 induces pro-LL-37 expression in myeloid precursors from patients with severe congenital neutropenia
J. Leukoc. Biol.,
November 1, 2008;
84(5):
1279 - 1286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Taschner, C. Koesters, B. Platzer, A. Jorgl, W. Ellmeier, T. Benesch, and H. Strobl
Down-regulation of RXR{alpha} expression is essential for neutrophil development from granulocyte/monocyte progenitors
Blood,
February 1, 2007;
109(3):
971 - 979.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Roig, E. Richer, F. Canonne-Hergaux, P. Gros, and M. F. M. Cellier
Regulation of NRAMP1 gene expression by 1{alpha},25-dihydroxy-vitamin D3 in HL-60 phagocytes
J. Leukoc. Biol.,
May 1, 2002;
71(5):
890 - 904.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grande, M. Montanari, E. Tagliafico, R. Manfredini, T. Z. Marani, M. Siena, E. Tenedini, A. Gallinelli, and S. Ferrari
Physiological levels of 1{alpha}, 25 dihydroxyvitamin D3 induce the monocytic commitment of CD34+ hematopoietic progenitors
J. Leukoc. Biol.,
April 1, 2002;
71(4):
641 - 651.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Piemonti, P. Monti, M. Sironi, P. Fraticelli, B. E. Leone, E. Dal Cin, P. Allavena, and V. Di Carlo
Vitamin D3 Affects Differentiation, Maturation, and Function of Human Monocyte-Derived Dendritic Cells
J. Immunol.,
May 1, 2000;
164(9):
4443 - 4451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Oehler, O. Majdic, W. F. Pickl, J. Stockl, E. Riedl, J. Drach, K. Rappersberger, K. Geissler, and W. Knapp
Neutrophil Granulocyte-committed Cells Can Be Driven to Acquire Dendritic Cell Characteristics
J. Exp. Med.,
April 6, 1998;
187(7):
1019 - 1028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
