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Human osteoblasts support human hematopoietic progenitor cells in vitro
bone marrow cultures
RS Taichman, MJ Reilly and SG Emerson
Department of Periodontics, School of Dentistry, University of Michigan,
Ann Arbor 48109-1078, USA.
Hematopoietic stem cell differentiation occurs in direct proximity to
osteoblasts within the bone marrow cavity. Despite this striking
affiliation, surprisingly little is known about the precise cellular and
molecular impact of osteoblasts on the bone marrow microenvironment.
Recently, we showed that human osteoblasts produce a variety of cytokine
mRNAs including granulocyte colony-stimulating factor,
granulocyte-macrophage colony-stimulating factor, and interleukin-6. We
examined here the ability of osteoblasts to support the development of
hematopoietic colonies from progenitors as well the ability to maintain
long-term culture-initiating cells (LTC-IC) in vitro. Examination of the
hematopoietic cells recovered after 2 weeks of culture showed that
osteoblasts support the maintenance of immature hematopoietic phenotypes.
In methylcellulose assays, osteoblasts stimulate the development of
hematopoietic colonies to a level at least 10-fold over controls from
progenitor cells. Using limiting dilutional bone marrow cultures, we
observed an activity produced by osteoblasts resulting in an threefold to
fourfold expansion of human LTC-IC and progenitor cells in vitro. Thus, the
presence of hematopoietic stem cells in close proximity to endosteal
surfaces in vivo may be due in part to a requirement for osteoblast-derived
products.
Volume 87,
Issue 2,
pp. 518-524,
01/15/1996
Copyright © 1996 by The American Society of Hematology
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