|
|
 Previous Article | Table of Contents | Next Article 
Ex vivo expansion of murine marrow cells with interleukin-3 (IL-3), IL- 6,
IL-11, and stem cell factor leads to impaired engraftment in irradiated
hosts
SO Peters, EL Kittler, HS Ramshaw and PJ Quesenberry
University of Massachusetts Cancer Center, Worcester 01605, USA.
In vitro incubation of bone marrow cells with cytokines has been used as an
approach to expand stem cells and to facilitate retroviral integration.
Expansion of hematopoietic progenitor cells has been monitored by different
in vitro assays and in a few instances by in vivo marrow renewal in
myeloablated hosts. This is the first report of studies, using two
competitive transplant models, in which cytokine- treated cells, obtained
from nonpretreated donors (eg, 5-fluorouracil), were competed with normal
cells. A basic assumption is that the expansion of progenitors assayed in
vitro as high- and low- proliferative potential colony-forming cells (HPP-
and LPP-CFCs) indicates an expansion of stem cells which will repopulate in
vivo. This study shows that culture of marrow cells with four cytokines
(stem cell factor, interleukin-3 [IL-3], IL-6, IL-11) induces significant
expansion and proliferation of HPP-CFC and LPP-CFC. Cell-cycle analysis
showed that these hematopoietic progenitors were induced to actively cell
cycle by culture with these cytokines. In the first competitive transplant
model, which uses Ly5.2/Ly5.1 congenic mice, cytokine- cultured Ly5.2 cells
competed with noncultured Ly5.1 cells led to 5% +/- 1% engraftment at 12
weeks and to 4% +/- 2% engraftment at 22 weeks posttransplantation for the
cytokine exposed cells. Noncultured Ly5.2 cells competed with cultured
Ly5.1 cells led to 70% +/- 1% engraftment at 12 weeks and to 93% +/- 2%
engraftment at 22 weeks posttransplantation. In the second model, which
uses BALB/c marrow of opposite genders, cultured male cells lead to 13% +/-
9% engraftment at 10 weeks and 2% +/- 1% engraftment at 14 weeks
posttransplantation; noncultured male cells lead to 70% +/- 2% and 95% +/-
2% engraftment at 10 and 14 weeks posttransplantation, respectively. Data
presented here from two different competitive transplant studies show a
defect of cytokine expanded marrow related to cell cycle activation which
manifests as defective long-term repopulating capability in irradiated host
mice. The engraftment defect is more profound at longer time intervals,
suggesting that the most striking effect may be on long-term repopulating
cells.
Volume 87,
Issue 1,
pp. 30-37,
01/01/1996
Copyright © 1996 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Miccio, R. Cesari, F. Lotti, C. Rossi, F. Sanvito, M. Ponzoni, S. J. E. Routledge, C.-M. Chow, M. N. Antoniou, and G. Ferrari
In vivo selection of genetically modified erythroblastic progenitors leads to long-term correction of {beta}-thalassemia
PNAS,
July 29, 2008;
105(30):
10547 - 10552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pearl-Yafe, J. Stein, E. S. Yolcu, D. L. Farkas, H. Shirwan, I. Yaniv, and N. Askenasy
Fas Transduces Dual Apoptotic and Trophic Signals in Hematopoietic Progenitors
Stem Cells,
December 1, 2007;
25(12):
3194 - 3203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Jung, J. Wang, J. Song, Y. Shiozawa, J. Wang, A. Havens, Z. Wang, Y.-X. Sun, S. G. Emerson, P. H. Krebsbach, et al.
Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation
Blood,
July 1, 2007;
110(1):
82 - 90.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ghiaur, A. Lee, J. Bailey, J. A. Cancelas, Y. Zheng, and D. A. Williams
Inhibition of RhoA GTPase activity enhances hematopoietic stem and progenitor cell proliferation and engraftment
Blood,
September 15, 2006;
108(6):
2087 - 2094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Nygren, D. Bryder, and S. E. W. Jacobsen
Prolonged Cell Cycle Transit Is a Defining and Developmentally Conserved Hemopoietic Stem Cell Property
J. Immunol.,
July 1, 2006;
177(1):
201 - 208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bonig, G. V. Priestley, and T. Papayannopoulou
Hierarchy of molecular-pathway usage in bone marrow homing and its shift by cytokines
Blood,
January 1, 2006;
107(1):
79 - 86.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. Srour, X. Tong, K. W. Sung, P. A. Plett, S. Rice, J. Daggy, C. T. Yiannoutsos, R. Abonour, and C. M. Orschell
Modulation of in vitro proliferation kinetics and primitive hematopoietic potential of individual human CD34+CD38-/lo cells in G0
Blood,
April 15, 2005;
105(8):
3109 - 3116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yang, I. Dybedal, D. Bryder, L. Nilsson, E. Sitnicka, Y. Sasaki, and S. E. W. Jacobsen
IFN-{gamma} Negatively Modulates Self-Renewal of Repopulating Human Hemopoietic Stem Cells
J. Immunol.,
January 15, 2005;
174(2):
752 - 757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Ivanovic, F. Hermitte, P. B. de la Grange, B. Dazey, F. Belloc, F. Lacombe, G. Vezon, and V. Praloran
Simultaneous Maintenance of Human Cord Blood SCID-Repopulating Cells and Expansion of Committed Progenitors at Low O2 Concentration (3%)
Stem Cells,
September 1, 2004;
22(5):
716 - 724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Ahmed, S. J. Ings, A. R. Pizzey, M. P. Blundell, A. J. Thrasher, H. T. Ye, A. Fahey, D. C. Linch, and K. L. Yong
Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model
Blood,
March 15, 2004;
103(6):
2079 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Stier, T. Cheng, R. Forkert, C. Lutz, D. M. Dombkowski, J. L. Zhang, and D. T. Scadden
Ex vivo targeting of p21Cip1/Waf1 permits relative expansion of human hematopoietic stem cells
Blood,
August 15, 2003;
102(4):
1260 - 1266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Geronimi, E. Richard, I. Redonnet-Vernhet, I. Lamrissi-Garcia, M. Lalanne, C. Ged, F. Moreau-Gaudry, and H. de Verneuil
Highly Efficient Lentiviral Gene Transfer in CD34+ and CD34+/38-/lin- Cells from Mobilized Peripheral Blood after Cytokine Prestimulation
Stem Cells,
July 1, 2003;
21(4):
472 - 480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Lambert, M. Liu, G. A. Colvin, M. Dooner, C. I. McAuliffe, P. S. Becker, B. G. Forget, S. M. Weissman, and P. J. Quesenberry
Marrow Stem Cells Shift Gene Expression and Engraftment Phenotype with Cell Cycle Transit
J. Exp. Med.,
June 2, 2003;
197(11):
1563 - 1572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry, G. A. Colvin, and J.-F. Lambert
The chiaroscuro stem cell: a unified stem cell theory
Blood,
December 15, 2002;
100(13):
4266 - 4271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, A. A. Saini, D. J. Chute, M. R. Wells, W. B. Clark, D. M. Harlan, J. Park, M. K. Stull, C. Civin, and T. A. Davis
Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow
Blood,
December 15, 2002;
100(13):
4433 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Josephson, G. Vassilopoulos, G. D. Trobridge, G. V. Priestley, B. L. Wood, T. Papayannopoulou, and D. W. Russell
Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors
PNAS,
June 11, 2002;
99(12):
8295 - 8300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Giet, D. R. Van Bockstaele, I. Di Stefano, S. Huygen, R. Greimers, Y. Beguin, and A. Gothot
Increased binding and defective migration across fibronectin of cycling hematopoietic progenitor cells
Blood,
March 15, 2002;
99(6):
2023 - 2031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bryder, V. Ramsfjell, I. Dybedal, K. Theilgaard-Monch, C.-M. Hogerkorp, J. Adolfsson, O. J. Borge, and S. E. W. Jacobsen
Self-Renewal of Multipotent Long-Term Repopulating Hematopoietic Stem Cells Is Negatively Regulated by Fas and Tumor Necrosis Factor Receptor Activation
J. Exp. Med.,
October 1, 2001;
194(7):
941 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Dybedal, D. Bryder, A. Fossum, L. S. Rusten, and S. E. W. Jacobsen
Tumor necrosis factor (TNF)-mediated activation of the p55 TNF receptor negatively regulates maintenance of cycling reconstituting human hematopoietic stem cells
Blood,
September 15, 2001;
98(6):
1782 - 1791.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Farese, W. G. Smith, J. G. Giri, N. Siegel, J. P. McKearn, and T. J. MacVittie
Promegapoietin-1a, an Engineered Chimeric IL-3 and Mpl-L Receptor Agonist, Stimulates Hematopoietic Recovery in Conventional and Abbreviated Schedules Following Radiation-Induced Myelosuppression in Nonhuman Primates
Stem Cells,
July 1, 2001;
19(4):
329 - 338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Sharma, M. C. Nelson, J. E. Brandt, M. Wessman, N. Mahmud, K. P. Weller, and R. Hoffman
Human CD34+ stem cells express the hiwi gene, a human homologue of the Drosophila gene piwi
Blood,
January 15, 2001;
97(2):
426 - 434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, I.-H. Oh, and C. J. Eaves
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0
Blood,
December 15, 2000;
96(13):
4185 - 4193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. S. Rosler, J. E. Brandt, J. Chute, and R. Hoffman
An in vivo competitive repopulation assay for various sources of human hematopoietic stem cells
Blood,
November 15, 2000;
96(10):
3414 - 3421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. M. Crooks, Q.-L. Hao, D. Petersen, L. W. Barsky, and D. Bockstoce
IL-3 Increases Production of B Lymphoid Progenitors from Human CD34+CD38- Cells
J. Immunol.,
September 1, 2000;
165(5):
2382 - 2389.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bryder and S. E. W. Jacobsen
Interleukin-3 supports expansion of long-term multilineage repopulating activity after multiple stem cell divisions in vitro
Blood,
September 1, 2000;
96(5):
1748 - 1755.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Orschell-Traycoff, K. Hiatt, R. N. Dagher, S. Rice, M. C. Yoder, and E. F. Srour
Homing and engraftment potential of Sca-1+lin- cells fractionated on the basis of adhesion molecule expression and position in cell cycle
Blood,
August 15, 2000;
96(4):
1380 - 1387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kirby, W. Walton, and O. Smithies
Hematopoietic stem cells with controllable tEpoR transgenes have a competitive advantage in bone marrow transplantation
Blood,
June 15, 2000;
95(12):
3710 - 3715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-J. Blouin, M. E. De Paepe, and M. Trudel
Altered Hematopoiesis in Murine Sickle Cell Disease
Blood,
August 15, 1999;
94(4):
1451 - 1459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bennaceur-Griscelli, C. Tourino, B. Izac, W. Vainchenker, and L. Coulombel
Murine Stromal Cells Counteract the Loss of Long-Term Culture-Initiating Cell Potential Induced by Cytokines in CD34+CD38low/neg Human Bone Marrow Cells
Blood,
July 15, 1999;
94(2):
529 - 538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Andrews, R. A. Briddell, R. Hill, M. Gough, and I. K. McNiece
Engraftment of Primates with G-CSF Mobilized Peripheral Blood CD34+ Progenitor Cells Expanded in G-CSF, SCF and MGDF Decreases the Duration and Severity of Neutropenia
Stem Cells,
July 1, 1999;
17(4):
210 - 218.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. H. Cowan, J. A. Moscow, H. Huang, J. A. Zujewski, J. O'Shaughnessy, B. Sorrentino, K. Hines, C. Carter, E. Schneider, G. Cusack, et al.
Paclitaxel Chemotherapy after Autologous Stem-Cell Transplantation and Engraftment of Hematopoietic Cells Transduced with a Retrovirus Containing the Multidrug Resistance Complementary DNA (MDR1) in Metastatic Breast Cancer Patients
Clin. Cancer Res.,
July 1, 1999;
5(7):
1619 - 1628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Moscow, H. Huang, C. Carter, K. Hines, J. Zujewski, G. Cusack, C. Chow, D. Venzon, B. Sorrentino, Y. Chiang, et al.
Engraftment of MDR1 and NeoR Gene-Transduced Hematopoietic Cells After Breast Cancer Chemotherapy
Blood,
July 1, 1999;
94(1):
52 - 61.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Brandt, A. M. Bartholomew, J. D. Fortman, M. C. Nelson, E. Bruno, L. M. Chen, J. V. Turian, T. A. Davis, J. P. Chute, and R. Hoffman
Ex Vivo Expansion of Autologous Bone Marrow CD34+ Cells With Porcine Microvascular Endothelial Cells Results in a Graft Capable of Rescuing Lethally Irradiated Baboons
Blood,
July 1, 1999;
94(1):
106 - 113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Möbest, S.-R. Goan, I. Junghahn, J. Winkler, I. Fichtner, M. Becker, E. de Lima-Hahn, R. Mertelsmann, and R. Henschler
Differential Kinetics of Primitive Hematopoietic Cells Assayed In Vitro and In Vivo During Serum-Free Suspension Culture of CD34+ Blood Progenitor Cells
Stem Cells,
May 1, 1999;
17(3):
152 - 161.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. S. Case, M. A. Price, C. T. Jordan, X. J. Yu, L. Wang, G. Bauer, D. L. Haas, D. Xu, R. Stripecke, L. Naldini, et al.
Stable transduction of quiescent CD34+CD38- human hematopoietic cells by HIV-1-based lentiviral vectors
PNAS,
March 16, 1999;
96(6):
2988 - 2993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, M. J. Bass, G. Van Zant, and B. Grimes
Organ-Selective Homing Defines Engraftment Kinetics of Murine Hematopoietic Stem Cells and Is Compromised by Ex Vivo Expansion
Blood,
March 1, 1999;
93(5):
1557 - 1566.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Guenechea, J.C. Segovia, B. Albella, M. Lamana, M. Ramirez, C. Regidor, M.N. Fernandez, and J.A. Bueren
Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo-Expanded Human CD34+ Cord Blood Cells
Blood,
February 1, 1999;
93(3):
1097 - 1105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Kennedy and J. L. Abkowitz
Mature monocytic cells enter tissues and engraft
PNAS,
December 8, 1998;
95(25):
14944 - 14949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gothot, J. C.M. van der Loo, D. W. Clapp, and E. F. Srour
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice
Blood,
October 15, 1998;
92(8):
2641 - 2649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Bunting, J. Galipeau, D. Topham, E. Benaim, and B. P. Sorrentino
Transduction of Murine Bone Marrow Cells With an MDR1 Vector Enables Ex Vivo Stem Cell Expansion, but These Expanded Grafts Cause a Myeloproliferative Syndrome in Transplanted Mice
Blood,
October 1, 1998;
92(7):
2269 - 2279.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
