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Bone marrow and splenic granulocyte-macrophage colony-stimulating factor
and transforming growth factor-beta mRNA levels in irradiated mice
CM Chang, A Limanni, WH Baker, ME Dobson, JF Kalinich, W Jackson and ML Patchen
Department of Experimental Hematology, Armed Forces Radiobiology Research
Institute, Bethesda, MD 20889-5603, USA.
The effects of a myeloablative sublethal 775 cGy 60C gamma radiation
exposure on endogenous bone marrow (BM) and splenic granulocyte- macrophage
colony-stimulating factor (GM-CSF) and transforming growth factor-beta
(TGF-beta) mRNA levels were assayed in B6D2F1 female mice. BM and spleen
were harvested from normal mice and irradiated mice on days 2, 4, 7, 10,
and 14 after exposure. Cytokine mRNA levels were determined using reverse
transcription-polymerase chain reaction. After irradiation, GM-CSF mRNA
levels were significantly increased in the BM from days 2 to 10 and in the
spleen from days 4 to 10. However, when BM and splenic GM-CSF protein
levels were measured using Western dot blot, no increased protein levels
were detected. Serum GM-CSF levels were likewise unchanged. Radiation
exposure did not affect BM or splenic TGF- beta mRNA levels and this
cytokine is known to be produced by cell populations similar to those that
produce GM-CSF. These data suggest that radiation injury to hemopoietic
tissues results in differential effects on GM-CSF and TGF-beta mRNA levels
and that, in the case of GM- CSF, increased mRNA levels are not matched by
increased protein production.
Volume 86,
Issue 6,
pp. 2130-2136,
09/15/1995
Copyright © 1995 by The American Society of Hematology
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