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Interindividual conservation of T-cell receptor beta chain variable regions
by minor histocompatibility antigen-specific HLA-A*0201- restricted
cytotoxic T-cell clones
E Goulmy, J Pool and PJ van den Elsen
Department of Immunohaematology and Blood Bank, University Hospital Leiden,
The Netherlands.
Minor histocompatibility antigens (mHags) are involved in the induction of
graft-versus-host disease (GVHD) after HLA-identical bone marrow
transplantation. Previously, we isolated a series of HLA-A*0201- restricted
cytotoxic T-cell (CTL) clones specific for the same mHag HA- 1 from
peripheral blood of three unrelated patients who were suffering from GVHD.
We have now analyzed the composition of the T-cell receptor (TCR) V regions
of 12 of these mHag HA-1-specific HLA-A*0201-restricted CTL clones by DNA
sequencing of the alpha and beta chains. Of these 12 clones, derived from
three unrelated individuals, five independent TCR alpha V- and beta
V-region sequences were established. The TCR alpha chains were composed of
varying TCR alpha V and TCR alpha J genes with no obvious similarities in
structure in the N regions. However, the TCR beta chains all used the TCR
beta V6S9 gene segment, and showed remarkable similarities within the N-D-N
regions; ie, three independent beta-chain sequences (originating from
donors Ha and Gy) shared a leucine/valine amino acid pair, whereas the
other two (originating from donors Ha and Wi) shared a serine/threonine
pair, all at positions 99 and 100 of the TCR beta V region. In conclusion,
the TCR analysis of HA- 1 mHag-specific CTL clones has shown that the HA-1
mHag/HLA-A*0201 complex selects for highly similar TCR beta V regions.
Volume 85,
Issue 9,
pp. 2478-2481,
05/01/1995
Copyright © 1995 by The American Society of Hematology
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