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Characterization of a new monoclonal antibody (PG-M3) directed against the
aminoterminal portion of the PML gene product: immunocytochemical evidence
for high expression of PML proteins on activated macrophages, endothelial
cells, and epithelia
L Flenghi, M Fagioli, L Tomassoni, S Pileri, M Gambacorta, R Pacini, F Grignani, T Casini, PF Ferrucci, MF Martelli, PG Pelicci, and B Falini
Institute of Hematology, University of Perugia, Italy.
PG-M3 is a new monoclonal antibody (MoAb) specifically directed against a
peptide sequence located in the aminoterminal region of the human PML
protein. PML gene fuses with the retinoic acid receptor alpha (RAR alpha)
gene during the t(15; 17) chromosomal translocation of acute promyelocytic
leukemia (APL). The epitope recognized by PG-M3 is species-specific and
fixative-resistant and is shared by most PML isoforms and PML/RAR alpha
fusion proteins. PML is consistently located within the nucleus, although a
minority of cells (about 20%), both in vitro and in vivo, show positivity
for PML also in the cytoplasm. The nuclear staining pattern of PG-M3 varies
from speckled (cells other than APL) to micropunctate (APL cells). Although
two physiologically expressed PML isoforms are detectable by
immunocytochemistry only or predominantly in the cytoplasm of transfected
cells, the cytoplasmic localization of PML is a property also shared by the
PML isoforms that predominantly localize to the nuclei. Immunohistologic
analysis of normal human tissues with the PG-M3 MoAb showed variable PML
expression, with the highest levels of the protein in postmitotic,
differentiated cell types, such as endothelial cells, epithelia, and tissue
macrophages, especially activated ones. In keeping with this in vivo
finding, PML appears strongly upregulated in the U937 promonocyte cell line
after exposure to agents that induce monocyte/macrophage activation
(interferon gamma) or maturation (vitamin D3 and transforming growth factor
beta 1).
Volume 85,
Issue 7,
pp. 1871-1880,
04/01/1995
Copyright © 1995 by The American Society of Hematology
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