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Leukocyte integrin CD11b promoter directs expression in lymphocytes and
granulocytes in transgenic mice
A Back, K East and D Hickstein
Medical Research Service, Seattle Veterans Affairs Medical Center, WA.
The human leukocyte integrin subunit CD11b is expressed predominantly on
myelomonocytic cells. To identify CD11b promoter sequences important for
myelomonocytic gene expression and to assess the utility of the CD11b
promoter for expressing heterologous genes in vivo, we generated transgenic
mice with a human CD4 reporter gene driven by CD11b promoter constructs
composed of 1.5, 0.3, or 0.1 kb of DNA sequence 5' to the transcription
start site. Using flow cytometry to detect the human CD4 reporter on murine
leukocytes, two of three 1.5-kb CD11b promoter founder lines showed surface
expression of the human CD4 transgene in granulocytes and lymphocytes. The
transgene expression observed in lymphocytes was inappropriate relative to
the normal pattern of CD11b expression. Of the eight 0.3-kb or 0.1-kb
founder lines, only one 0.1- kb founder line showed transgene expression.
The overall pattern of transgene expression among the 11 founder lines does
not parallel expression of the endogenous CD11b gene. These studies
indicate that additional CD11b regulatory elements will be required to
express a reporter gene in vivo in a lineage-specific pattern that mimics
the endogenous CD11b gene.
Volume 85,
Issue 4,
pp. 1017-1024,
02/15/1995
Copyright © 1995 by The American Society of Hematology
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