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Adult bone marrow-derived pluripotent hematopoietic stem cells are
engraftable when transferred in utero into moderately anemic fetal
recipients
BR Blazar, PA Taylor and DA Vallera
Department of Pediatrics, and Therapeutic Radiology, University of
Minnesota Hospital and Clinics, Minneapolis.
We have used W41/W41 (C57BL/6-Ly 5.1, Gpi-1b) anemic mice and a newly
developed double congenic donor strain (C57BL/6-Ly 5.2, Gpi-1a) to
determine if adult bone marrow (BM) injected in utero could provide stem
cell engraftment. Of 38 fetuses injected intraperitoneally on day 13/14 of
gestation with donor BM cells, 17 (47%) were live-born. On day 6, 12% had
erythroid engraftment. On day 59, in 50% (8/16) of mice, 50% to 75% of
erythroid cells, 42% of T cells, 5% of B cells, and 26% of granulocytes in
the peripheral blood (PB) were derived from the in utero-injected donor BM.
At 141 days, thymic, splenic, lymph node, BM, and PB chimerism studies
showed that 57% to 80% of T cells, 10% to 15% of B cells, and 27% to 43% of
granulocytes were of donor origin. At this time, BM was injected into
irradiated secondary recipients. On day 104 posttransfer, a mean 23% of T
cells, 8% of B cells, and 40% of granulocytes were derived from the in
utero donor BM. These data indicate that adult BM has hierarchical
engraftment capabilities in W41/W41 mice and prove that stem cells are
engraftable in utero.
Volume 85,
Issue 3,
pp. 833-841,
02/01/1995
Copyright © 1995 by The American Society of Hematology
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