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The CD11b promoter directs high-level expression of reporter genes in
macrophages in transgenic mice [published erratum appears in Blood 1995 Apr
1;85(7):1983]
S Dziennis, RA Van Etten, HL Pahl, DL Morris, TL Rothstein, CM Blosch, RM Perlmutter and DG Tenen
Department of Medicine, Beth Israel Hospital, Boston, MA 02215.
CD11b is the alpha chain of the Mac-1 integrin and is preferentially
expressed in myeloid cells (neutrophils, monocytes, and macrophages). We
have previously shown that the CD11b promoter directs cell-type- specific
expression in myeloid lines using transient transfection assays. To confirm
that these promoter sequences contain the proper regulatory elements for
correct myeloid expression of CD11b in vivo, we have used the -1.7-kb human
CD11b promoter to direct reporter gene expression in transgenic mice.
Stable founder lines were generated with two different reporter genes, a
Thy 1.1 surface marker and the Escherichia coli lacZ (beta-galactosidase)
gene. Analysis of founders generated with each reporter demonstrated that
the CD11b promoter was capable of driving high levels of transgene
expression in murine macrophages for the lifetime of the animals. Similar
to the endogenous gene, transgene expression was preferentially found in
mature monocytes, macrophages, and neutrophils and not in myeloid
precursors. These experiments indicate that the -1.7 CD11b promoter
contains the regulatory elements sufficient for high-level macrophage
expression. This promoter should be useful for targeting heterologous gene
expression to mature myeloid cells.
Volume 85,
Issue 2,
pp. 319-329,
01/15/1995
Copyright © 1995 by The American Society of Hematology
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