Lymphohematopoietic progenitors of normal mice
TC Ball, F Hirayama and M Ogawa
Department of Medicine, Medical University of South Carolina, Charleston,
USA.
We have identified and characterized the lymphohematopoietic progenitors in
the bone marrow of normal mice using a single-step methylcellulose culture
assay. Lineage-negative Ly-6A/E (Sca-1)+ progenitors isolated from normal
mice were plated in methylcellulose culture containing steel factor (SF),
interleukin-7 (IL-7), erythropoietin (Ep), and IL-11. After 16 to 17 days
of culture, pre-B- cell-containing multilineage myeloid colonies can be
microscopically identified; however, flow-cytometric analysis of individual
colonies for B220-positive cells proved superior to in situ microscopic
identification of lymphomyeloid colonies. Approximately 10% (6/66) of the
mixed colonies without a conspicuous B-cell component had B220- positive
cells. The single cell origin of the lymphomyeloid colonies was confirmed
by micromanipulation. Although the combination of SF, IL- 7, and Ep was
sufficient to support formation of lymphomyeloid colonies, addition of
IL-11, granulocyte colony-stimulating factor or IL-12 to the combination of
SF, IL-7, and Ep increased the number of lymphomyeloid colonies. IL-1 alpha
and IL-3 independently inhibited the expression of the B-lymphoid lineage
when added to the combination of SF, IL-7, Ep, and IL-11. Approximately
four times more lymphohematopoietic progenitors are present in normal mice
than in mice treated with 5-fluorouracil.
Volume 85,
Issue 11,
pp. 3086-3092,
06/01/1995
Copyright © 1995 by The American Society of Hematology
