|
|
 Previous Article | Table of Contents | Next Article 
Full-length but not truncated CD34 inhibits hematopoietic cell
differentiation of M1 cells
MJ Fackler, DS Krause, OM Smith, CI Civin and WS May
Division of Hematologic Malignancies, Johns Hopkins Oncology Center,
Baltimore, MD 21231, USA.
CD34 is expressed on human and murine hematopoietic stem and progenitor
cells and its clinical usefulness for isolation of stem/progenitor cells
has been well established. Although expression of CD34 is regulated in a
developmental stage-specific manner, the function of CD34 is not known.
Recently we have shown that both a full-length and truncated form of CD34
protein is expressed by hematopoietic cells (Blood 84:691, 1994). To test
whether failure to suppress either form of CD34 could affect terminal
myeloid differentiation, we constitutively expressed these CD34 proteins in
murine M1 myeloid leukemia cells, which can be terminally differentiated to
macrophages by treatment with interleukin-6 of leukemia inhibitory factor.
Surprisingly our results show that forced expression of the full-length but
not the truncated form of CD34 impedes terminal differentiation by these
agents. Because the difference between the two forms of CD34 protein
resides in the length of their respective cytoplasmic tail domains, our
findings strongly suggest that the cytoplasmic domain region of full-length
CD34 is responsible for the observed maturation arrest phenotype. These
findings suggest a potential negative regulatory role for full-length CD34
in hematopoietic cell differentiation and may explain, at least in part,
the block in maturation observed in CD34+ acute myeloid leukemia.
Volume 85,
Issue 11,
pp. 3040-3047,
06/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. S. Nielsen and K. M. McNagny
Novel functions of the CD34 family
J. Cell Sci.,
November 15, 2008;
121(22):
3683 - 3692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Jiang, P. S. E. Yue, D. Drenkard, and H. Schwarz
Induction of Proliferation and Monocytic Differentiation of Human CD34+ Cells by CD137 Ligand Signaling
Stem Cells,
September 1, 2008;
26(9):
2372 - 2381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Acevedo, I. Londono, M. Oubaha, L. Ghitescu, and M. Bendayan
Glomerular CD34 Expression in Short- and Long-term Diabetes
J. Histochem. Cytochem.,
June 1, 2008;
56(6):
605 - 614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Medina, R. T. Kilani, N. Carr, E. Brown, and A. Ghahary
Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells
Am. J. Pathol.,
October 1, 2007;
171(4):
1140 - 1152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. G. Wang, M. P. Pasillas, and M. P. Kamps
Meis1 programs transcription of FLT3 and cancer stem cell character, using a mechanism that requires interaction with Pbx and a novel function of the Meis1 C-terminus
Blood,
July 1, 2005;
106(1):
254 - 264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Murga, L. Yao, and G. Tosato
Derivation of Endothelial Cells from CD34- Umbilical Cord Blood
Stem Cells,
May 1, 2004;
22(3):
385 - 395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Richardson, R. Starr, L. J. L. Angus, and D. J. Hilton
A Somatic Cell Genetic System for Dissecting Hemopoietic Cytokine Signal Transduction
J. Biol. Chem.,
July 5, 2002;
277(28):
25624 - 25630.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-N. Lee, J.-S. Kang, and R. S. Krauss
Identification of a Role for the Sialomucin CD164 in Myogenic Differentiation by Signal Sequence Trapping in Yeast
Mol. Cell. Biol.,
November 15, 2001;
21(22):
7696 - 7706.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Felschow, M. L. McVeigh, G. T. Hoehn, C. I. Civin, and M. J. Fackler
The adapter protein CrkL associates with CD34
Blood,
June 15, 2001;
97(12):
3768 - 3775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Prosper and C. M. Verfaillie
Regulation of hematopoiesis through adhesion receptors
J. Leukoc. Biol.,
March 1, 2001;
69(3):
307 - 316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. R. Beauchamp, L. Heslop, D. S.W. Yu, S. Tajbakhsh, R. G. Kelly, A. Wernig, M. E. Buckingham, T. A. Partridge, and P. S. Zammit
Expression of CD34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells
J. Cell Biol.,
December 11, 2000;
151(6):
1221 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Huss, C. Lange, E. M. Weissinger, H.-J. Kolb, and K. Thalmeier
Evidence of Peripheral Blood-Derived, Plastic-Adherent CD34-/low Hematopoietic Stem Cell Clones with Mesenchymal Stem Cell Characteristics
Stem Cells,
July 1, 2000;
18(4):
252 - 260.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. M. Watt, L. H. Butler, M. Tavian, H.-J. Buhring, I. Rappold, P. J. Simmons, A. C. W. Zannettino, D. Buck, A. Fuchs, R. Doyonnas, et al.
Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues
Blood,
May 15, 2000;
95(10):
3113 - 3124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Sassetti, A. Van Zante, and S. D. Rosen
Identification of Endoglycan, a Member of the CD34/Podocalyxin Family of Sialomucins
J. Biol. Chem.,
March 17, 2000;
275(12):
9001 - 9010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wang, H. Fjerdingstad, I. Strøm-Gundersen, and H. B. Benestad
Maturation Rate of Mouse Neutrophilic Granulocytes: Acceleration by Retardation of Proliferation, but No Detectable Influence from G-CSF or Stromal Cells
Stem Cells,
September 1, 1999;
17(5):
253 - 264.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J.-i. Tada, M. Omine, T. Suda, and N. Yamaguchi
A Common Signaling Pathway Via Syk and Lyn Tyrosine Kinases Generated From Capping of the Sialomucins CD34 and CD43 in Immature Hematopoietic Cells
Blood,
June 1, 1999;
93(11):
3723 - 3735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-Q. Liu, R. Ilaria Jr., P. D. Kingsley, A. Iwama, R. A. van Etten, J. Palis, and D.-E. Zhang
A Novel Ubiquitin-Specific Protease, UBP43, Cloned from Leukemia Fusion Protein AML1-ETO-Expressing Mice, Functions in Hematopoietic Cell Differentiation
Mol. Cell. Biol.,
April 1, 1999;
19(4):
3029 - 3038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.M. Verfaillie
Adhesion Receptors as Regulators of the Hematopoietic Process
Blood,
October 15, 1998;
92(8):
2609 - 2612.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C.W. Zannettino, H.-J. Buhring, S. Niutta, S. M. Watt, M. A. Benton, and P. J. Simmons
The Sialomucin CD164 (MGC-24v) Is an Adhesive Glycoprotein Expressed by Human Hematopoietic Progenitors and Bone Marrow Stromal Cells That Serves as a Potent Negative Regulator of Hematopoiesis
Blood,
October 15, 1998;
92(8):
2613 - 2628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Watt, H.-J. Buhring, I. Rappold, J. Y.-H. Chan, J. Lee-Prudhoe, T. Jones, A. C.W. Zannettino, P. J. Simmons, R. Doyonnas, D. Sheer, et al.
CD164, a Novel Sialomucin on CD34+ and Erythroid Subsets, Is Located on Human Chromosome 6q21
Blood,
August 1, 1998;
92(3):
849 - 866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C.-T. Hu and S. L. Chien
The Cytoplasmic Domain of Stem Cell Antigen CD34 Is Essential for Cytoadhesion Signaling But Not Sufficient for Proliferation Signaling
Blood,
February 15, 1998;
91(4):
1152 - 1162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. B. Wood, G. May, L. Healy, T. Enver, and G. M. Morriss-Kay
CD34 Expression Patterns During Early Mouse Development Are Related to Modes of Blood Vessel Formation and Reveal Additional Sites of Hematopoiesis
Blood,
September 15, 1997;
90(6):
2300 - 2311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y.-H. Chan, J. E. Lee-Prudhoe, B. Jorgensen, G. Ihrke, R. Doyonnas, A. C. W. Zannettino, V. J. Buckle, C. J. Ward, P. J. Simmons, and S. M. Watt
Relationship between Novel Isoforms, Functionally Important Domains, and Subcellular Distribution of CD164/Endolyn
J. Biol. Chem.,
January 12, 2001;
276(3):
2139 - 2152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
