SBH-1, a novel Reed-Sternberg-like cell line capable of inducing tumors in
SCID mice: immunophenotypic, cytogenetic, and cytokine expression profiles
JF DeCoteau, MD Reis, H Griesser, A Lorenzana, I al-Hashmi, RG Hawley, A Naumov, CA White and PH Pinkerton
Department of Laboratory Medicine, Sunnybrook Health Science Centre,
Toronto, Ontario, Canada.
A new cell line, SBH-1, with the morphologic, immunophenotypic, and
karyotypic features consistent with those of Reed-Sternberg (RS) and
Hodgkin (H) cells, has been established from the pleural effusion of a
patient. The cytologic appearance of SBH-1 cells is characteristic of
multinucleate RS and mononuclear H cells, all containing inclusion-like
nucleoli. The SBH-1 cells express CD30, CD15, CD25, CD71, CD45, CD20, CD22,
and bcl-2 protein and are negative for epithelial membrane antigen.
Cytogenetic analysis showed multiple clonal abnormalities with breakpoints
at 14q32, 6q21, and 11q23. The Ig heavy chain genes and both Ig light chain
genes were rearranged in SBH-1 cells, whereas the bcl-2 gene was in
germline configuration. Messages for the cytokines interleukin-1 beta (IL-1
beta), tumor necrosis factor-alpha, and transforming growth factor-beta and
the cytokine receptors IL-2R, IL- 4R, IL-6R, and IL-7R were detected by
reverse transcription-polymerase chain reaction analysis.
Xenotransplantation of SBH-1 cells into severe combined immunodeficient
(SCID) mice led to local and disseminated tumor growth. The cytologic,
histologic, and immunohistochemical features of SBH-1 cells in SCID mouse
tumors were typical of RS and H cells. The SBH-1 cell line will be useful
in the study of RS and H cell biology, inasmuch as it represents a cell
line obtained from a previously untreated patient.
Volume 85,
Issue 10,
pp. 2829-2838,
05/15/1995
Copyright © 1995 by The American Society of Hematology
