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Functional expression of adhesion receptors and costimulatory molecules by
fresh and immortalized B-cell non-Hodgkin's lymphoma cells
FA Vyth-Dreese, TA Dellemijn, JW van Oostveen, CA Feltkamp and A Hekman
Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Peripheral blood lymphocytes of a patient with follicular B-cell non-
Hodgkin's lymphoma (B-NHL) were immortalized in vitro by Epstein-Barr virus
(EBV). Eight cell lines were obtained (termed BNS1, BNS2-1 through BNS2-7),
which showed a pattern of idiotypic (id) Ig surface expression and Ig JH
and kappa gene rearrangement, identical to that of the parent cells (termed
NS), confirming their neoplastic origin. Induction of allogeneic T-cell
proliferation by NS cells was mediated by HLA-DR, leukocyte
function-associated antigen-1 (LFA-1), LFA-3, B7- 1/CD80, and CTLA4 and
resulted in the upregulation (LFA-3, intercellular adhesion molecule-1
[ICAM-1], CD40) and induction (B7- 1/CD80, B7-2/CD86, L16/activated LFA-1)
of accessory molecules on NS cells. In turn, responder T lymphocytes were
induced to express B7- 1/CD80, B7-2/CD86, CD40 ligand (CD40L), ICAM-1,
L16/activated LFA-1, and HLA-DR, reflecting bidirectional signaling between
T lymphocytes and B-NHL cells. Preactivation of NS cells by EBV
transformation or CD40 engagement resulted in enhanced expression of
accessory molecules and abolished the requirement for accessory cells
during allostimulation. These resting and activated clonal B cells will be
useful in further dissecting the requirements for B-NHL costimulation.
Volume 85,
Issue 10,
pp. 2802-2812,
05/15/1995
Copyright © 1995 by The American Society of Hematology
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