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Overexpression of the HOX4A (HOXD3) homeobox gene in human erythroleukemia
HEL cells results in altered adhesive properties
Y Taniguchi, N Komatsu and T Moriuchi
Department of Molecular Life Science (Cell Biology), Tokai University
School of Medicine, Kanagawa, Japan.
We previously isolated the human homeobox gene HOX4A (HOXD3) on chromosome
2 from a human genomic library and determined its nucleotide sequence. In
the present study, expression of the HOX4A gene was investigated in human
hematopoietic cell lines. Reverse transcriptase- mediated polymerase chain
reaction analysis showed that the HOX4A gene was expressed in
erythroleukemia HEL and K562 cells but not in promyelocytic leukemia HL-60
cells. To study the role of the HOX4A gene in erythropoiesis, expression
vectors containing the HOX4A gene in the sense or antisense orientation
were introduced into HEL cells. The sense transfectants overexpressing the
HOX4A gene formed aggregates, which were composed of densely associated
cells adhering to tissue- culture dishes, whereas the parental HEL cells
and antisense transfectants adhered poorly to the dishes. Furthermore, the
sense transfectants overexpressing the HOX4A gene attached more efficiently
to fibronectin and collagen than did the antisense transfectants and
parental HEL cells. Northern blot analysis showed that integrin beta 3 mRNA
levels were significantly increased in the HEL cells overexpressing the
HOX4A gene, whereas the integrin beta 1 and alpha IIb mRNA levels did not
show a distinct correlation with HOX4A mRNA levels. Fluorescence-activated
cell sorting analysis showed that the sense transfectants overexpressing
the HOX4A gene expressed increased levels of integrin alpha IIb beta 3 (GP
IIb-IIIa) complex as compared with the parental HEL cells and antisense
transfectants. These results implicate the homeobox gene HOX4A in the
regulation of cell adhesion processes.
Volume 85,
Issue 10,
pp. 2786-2794,
05/15/1995
Copyright © 1995 by The American Society of Hematology
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