|
|
 Previous Article | Table of Contents | Next Article 
Cyclic guanosine monophosphate-dependent protein kinase is targeted to
intermediate filaments and phosphorylates vimentin in A23187-stimulated
human neutrophils
KB Pryzwansky, TA Wyatt and TM Lincoln
Department of Pathology, University of North Carolina, Chapel Hill
27599-7525.
The effects of the calcium ionophore, A23187, on human neutrophil
activation were studied in relation to the signaling mechanism of cyclic
guanosine monophosphate (cGMP)-dependent protein kinase (G- kinase).
Immunocytochemistry demonstrated that G-kinase translocated from a diffuse
localization in the cytoplasm to the cytoskeleton after stimulation with
A23187. Over a period of 5 minutes, G-kinase was transiently colocalized
with the intermediate filament protein, vimentin. At 3 minutes' stimulation
with A23187, colocalization of G- kinase and vimentin was predominantly
confined to filaments that extended into the uropod. The time of
colocalization of G-kinase and vimentin was reduced in the
A23187-stimulated cell from 3 minutes to 1 minute by 8-Br-cGMP. Coincident
with colocalization was an increase in cGMP levels and transient
phosphorylation of vimentin in adhered A23187- stimulated cells.
Phosphorylation of vimentin was maximal after 3 minutes with A23187, and
was essentially over at 5 minutes. The time of phosphorylation of vimentin
was also reduced from 3 minutes to 1 minute when cells were preincubated
with 8-Br-cGMP and then stimulated with A23187, which suggests that cyclic
adenosine monophosphate (cAMP)- dependent protein kinase does not
phosphorylate vimentin in A23187- treated neutrophils. Phosphorylation of
vimentin was not observed in nonactivated cells treated only with
8-Br-cGMP. The presence of the protein kinase C inhibitors, staurosporine
or H-7, did not inhibit vimentin phosphorylation in A23187-treated cells,
which provides supportive data that protein kinase C is not the
phosphorylating enzyme. These results suggest that vimentin and G-kinase
are colocalized in a Ca(2+)-dependent manner in neutrophils, and that
vimentin is transiently phosphorylated by G-kinase in response to the
colocalization of the two proteins. The transient redistribution of
compartmentalized G-kinase represents one type of neutrophil activation
mechanism.
Volume 85,
Issue 1,
pp. 222-230,
01/01/1995
Copyright © 1995 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. G. Werner, V. Godfrey, R. R. Arnold, G. L. Featherstone, D. Bender, J. Schlossmann, M. Schiemann, F. Hofmann, and K. B. Pryzwansky
Neutrophil Dysfunction in Guanosine 3',5'-Cyclic Monophosphate-Dependent Protein Kinase I-Deficient Mice
J. Immunol.,
August 1, 2005;
175(3):
1919 - 1929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hou, J. Lascola, N. O. Dulin, R. D. Ye, and D. D. Browning
Activation of cGMP-dependent Protein Kinase by Protein Kinase C
J. Biol. Chem.,
May 2, 2003;
278(19):
16706 - 16712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Chen, R. M. McCarron, S. Golech, J. Bembry, B. Ford, F. A. Lenz, N. Azzam, and M. Spatz
ET-1- and NO-mediated signal transduction pathway in human brain capillary endothelial cells
Am J Physiol Cell Physiol,
February 1, 2003;
284(2):
C243 - C249.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Hennan and J. Diamond
Effect of NO donors on protein phosphorylation in intact vascular and nonvascular smooth muscles
Am J Physiol Heart Circ Physiol,
April 1, 2001;
280(4):
H1565 - H1580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-s. Gao and E. Sztul
A Novel Interaction of the Golgi Complex with the Vimentin Intermediate Filament Cytoskeleton
J. Cell Biol.,
February 26, 2001;
152(5):
877 - 894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Lucas, G. M. Pitari, S. Kazerounian, I. Ruiz-Stewart, J. Park, S. Schulz, K. P. Chepenik, and S. A. Waldman
Guanylyl Cyclases and Signaling by Cyclic GMP
Pharmacol. Rev.,
September 1, 2000;
52(3):
375 - 414.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Collins and M. D. Uhler
Cyclic AMP- and Cyclic GMP-dependent Protein Kinases Differ in Their Regulation of Cyclic AMP Response Element-dependent Gene Transcription
J. Biol. Chem.,
March 26, 1999;
274(13):
8391 - 8404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. D. Browning, N. D. Windes, and R. D. Ye
Activation of p38 Mitogen-activated Protein Kinase by Lipopolysaccharide in Human Neutrophils Requires Nitric Oxide-dependent cGMP Accumulation
J. Biol. Chem.,
January 1, 1999;
274(1):
537 - 542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. PETTIT
Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis
Physiol Rev,
October 1, 1998;
78(4):
949 - 967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. B. Pryzwansky and E. P. Merricks
Chemotactic Peptide-induced Changes of Intermediate Filament Organization in Neutrophils during Granule Secretion: Role of Cyclic Guanosine Monophosphate
Mol. Biol. Cell,
October 1, 1998;
9(10):
2933 - 2947.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. I. Patel, J. K. Hennan, and J. Diamond
Activation of Guanosine 3',5'-Cyclic Monophosphate (cGMP)-Dependent Protein Kinase in Rat Vas Deferens and Distal Colon is Not Accompanied by Inhibition of Contraction
J. Pharmacol. Exp. Ther.,
November 1, 1997;
283(2):
894 - 900.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. L. Foster, G. C. Higgins, and F. R. Jackson
Biochemical Properties and Cellular Localization of the Drosophila DG1 cGMP-dependent Protein Kinase
J. Biol. Chem.,
September 20, 1996;
271(38):
23322 - 23328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Murphy-Ullrich, M. Pallero, N Boerth, J. Greenwood, T. Lincoln, and T. Cornwell
Cyclic GMP-dependent protein kinase is required for thrombospondin and tenascin mediated focal adhesion disassembly
J. Cell Sci.,
January 10, 1996;
109(10):
2499 - 2508.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. L. Russell, D. Cao, D. Zhang, R. E. Handschumacher, and G. Pizzorno
Uridine Phosphorylase Association with Vimentin. INTRACELLULAR DISTRIBUTION AND LOCALIZATION
J. Biol. Chem.,
April 13, 2001;
276(16):
13302 - 13307.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
