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Steel factor affects SCL expression during normal erythroid differentiation
BA Miller, J Floros, JY Cheung, DM Wojchowski, L Bell, CG Begley, NJ Elwood, J Kreider and C Christian
Department of Pediatrics, Milton S. Hershey Medical Center, Pennsylvania
State University, Hershey 17033.
Steel factor is one of the growth factors that controls the proliferation
and differentiation of hematopoietic cells and SCL, also known as Tcl-5 or
Tal-1, is a transcription factor involved in erythropoiesis. In this
report, we studied the role of SCL in the proliferation of human peripheral
blood burst-forming unit-erythroid (BFU-E) and the effects of Steel factor
on SCL expression in proliferating erythroid cells. BFU-E-derived colonies
increase progressively in size, as determined by cell number, from day 7 to
day 14 of culture, with the greatest increase in colony size (10-fold
expansion) occurring between day 7 and day 10. SCL protein levels in
BFU-E-derived cells were highest in day 7 cells and decreased progressively
from day 7 to day 14 of culture, suggesting an association of SCL with
erythroid proliferation. In contrast, SCL mRNA levels did not decrease
significantly between day 7 and day 14 cells, suggesting that
posttranscriptional mechanisms are largely responsible for the decrease in
SCL protein observed. The role of SCL in Steel factor-induced erythroid
proliferation was then examined. In BFU-E- derived colonies cultured with
Steel factor, colony size was significantly increased compared to control.
In day 7 and day 10 erythroid precursors cultured with Steel factor, SCL
protein was increased significantly compared to control. The increase in
SCL protein levels in early erythroid precursors stimulated with Steel
factor suggests one mechanism through which Steel factor may enhance normal
erythroid proliferation. SCL mRNA levels assessed by Northern blot in day 7
cells did not increase significantly in response to Steel factor
stimulation, suggesting that posttranscriptional mechanisms may also be
important in the increase in SCL protein observed in response to Steel.
Volume 84,
Issue 9,
pp. 2971-2976,
11/01/1994
Copyright © 1994 by The American Society of Hematology
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