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 Previous Article | Table of Contents | Next Article 
Expression of the interleukin-6 (IL-6), IL-6 receptor, and gp130 genes in
acute leukemia
K Inoue, H Sugiyama, H Ogawa, T Yamagami, T Azuma, Y Oka, H Miwa, K Kita, A Hiraoka and T Masaoka
Department of Medicine III, Osaka University Medical School, Japan.
Expression patterns of interleukin-6 (IL-6), IL-6 receptor (IL-6R), and
gp130 genes in 39 patients with acute myeloid leukemia (AML), in 23
patients with acute lymphoblastic leukemia (ALL), and in 7 patients with
acute mixed lineage leukemia (AMLL) were studied by quantitative reverse
transcriptase-polymerase chain reaction. Significant levels of IL-6 were
expressed in 8 (21%) of 39 AML patients and in 2 (29%) of 7 AMLL patients,
whereas in ALL, the expression of IL-6 was almost negligible. IL-6R was
expressed in all patients with AML and AMLL, whereas only half of ALL
patients expressed low levels of IL-6R as compared with those with AML and
AMLL. However, gp130 was ubiquitously expressed in all the leukemia
patients, and there was no significant difference in gp130 expression among
AML, ALL, and AMLL. Significant correlation was observed between the
expression of IL-6R and gp130 in AML. When tested for in vitro response to
IL-6, the leukemic cells from 3 of 7 AML, none of 3 ALL, and both of 2 AMLL
patients significantly responded to IL-6, showing the correlation between
the expression levels of IL-6R and gp130 and the responsiveness of leukemic
cells to IL-6. These results showed that quantitation of IL-6R and gp130
expression by reverse transcriptase-polymerase chain reaction is useful for
the rapid prediction of the responsiveness of leukemic cells to IL- 6,
especially in cases of administration of IL-6.
Volume 84,
Issue 8,
pp. 2672-2680,
10/15/1994
Copyright © 1994 by The American Society of Hematology
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