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Human T-cell leukemia virus type I tax/rex DNA and RNA in cutaneous T- cell
lymphoma
SK Ghosh, JT Abrams, H Terunuma, EC Vonderheid and E DeFreitas
Department of Medicine, University of Miami, School of Medicine, FL 33101.
Peripheral blood mononuclear cells (PBMCs) and T-cell lines from patients
with Sezary syndrome (SS) and skin lesions from patients with mycosis
fungoides (MF) were examined by polymerase chain reaction (PCR) for DNA
sequences homologous to the human retroviruses human T- lymphotropic virus
(HTLV)-I and -II. Results obtained using primers and probes from the
tax/rex region of HTLV-I indicate that 72% (18/25) of SS patients PBMCs,
80% (20/25) of T-cell lines established from SS- PBMC, and 30% (3/10) of
skin lesions from MF patients were positive for HTLV-I tax/rex region DNA.
Sequence analysis of the 127-bp fragment amplified by the tax/rex primers
from 4 of these individuals was found to be identical to that in prototypic
HTLV-I. Negative results were obtained using primers and probes from the
HTLV-I gag region and the HTLV-II gag and tax regions. No PCR products were
obtained using all primers and probes using DNA from 9 healthy blood donors
and 10 cord bloods. Expression of HTLV-I tax/rex mRNA was found in 4 of 8
Sezary patients, as determined by RNA-PCR, indicating that this viral
region is being transcribed in vivo. Exposure to Tax/Rex protein in SS-
patients is supported by the fact that serum antibodies against p27rex and
p40tax was observed in 43% and 29% of these SS patients, respectively.
Although the causal relationship between the HTLV-I tax/rex region and
cutaneous T-cell lymphoma (CTCL) remains unclear, these findings support
the presence of a truncated HTLV-I retrovirus in CTCL patients.
Volume 84,
Issue 8,
pp. 2663-2671,
10/15/1994
Copyright © 1994 by The American Society of Hematology
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