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P2Z adenosine triphosphate receptor activity in cultured human monocyte-
derived macrophages
SE Hickman, J el Khoury, S Greenberg, I Schieren and SC Silverstein
Department of Physiology and Cellular Biophysics, Columbia University,
College of Physicians and Surgeons, New York, NY 10032.
The present study shows that human mononuclear phagocytes express a P2Z-
like purinergic membrane receptor activity. Extracellular adenosine
triphosphate (ATP) induces the formation of nonselective membrane pores in
human mononuclear phagocytes that allow the entry of otherwise membrane
impermeant fluorescent dyes (YO-PRO-1 or Lucifer yellow) into the cytoplasm
of these cells. The percentage of mononuclear phagocytes that was
permeabilized by ATP increased as monocytes matured into macrophages. Their
response to ATP was inhibited by Mg2+ and oxidized ATP. Benzoylbenzoic-ATP
(BzBzATP) was approximately 60% as effective as ATP and adenosine-5
-O-(thiophosphate) (ATP gamma S) was less than 20% as effective as ATP in
permeabilizing human macrophages to YO-PRO-1 or Lucifer Yellow. Thus, the
human P2Z-like receptor differs from its murine counterpart because
BzBzATP, ATP, and ATP gamma S are equally efficacious in permeabilizing
murine macrophage-like J774 cells to these dyes. UTP, GTP, and CTP were
ineffective in permeabilizing human or murine macrophages to YO-PRO-1.
Taken together, these data indicate that human monocyte-derived macrophages
express a P2Z-like activity that is pharmacologically distinct from that
expressed by their murine counterparts and that expression of these
receptors is developmentally regulated in human mononuclear phagocytes.
Volume 84,
Issue 8,
pp. 2452-2456,
10/15/1994
Copyright © 1994 by The American Society of Hematology
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