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Persistence of the same viral strain in early and late relapses of
Epstein-Barr virus-associated Hodgkin's disease
P Brousset, D Schlaifer, F Meggetto, E Bachmann, S Rothenberger, J Pris, G Delsol and H Knecht
Laboratoire d'Anatomie Pathologique, Centre Hospitalier Universitaire de
Purpan, Toulouse, France.
Twelve cases of relapsing Hodgkin's disease were investigated for the
presence of Epstein-Barr virus (EBV). Of these, 7 cases contained EBV gene
products (LMP1, EBER RNA) in the diagnostic Reed-Sternberg cells and
variants at first presentation and at relapse(s), whereas 5 cases were
negative at both first diagnosis and relapse. Among the 7 EBV- positive
cases, material for DNA extraction was available in 2 cases at both
diagnosis and relapse(s). Ig and T-cell receptor gene rearrangements
displayed a germline configuration in the 2 cases. However, Southern blot
analysis of the terminal repeats (TR) of EBV genome showed that, in 1 of
the 2 cases, the fragment was of the same size at diagnosis and in the
subsequent two relapses (1 early and 1 late). The second case contained
monoclonal EBV genome at diagnosis, but the Southern analysis of the TR was
negative at relapse. The latent membrane protein (LMP1) sequence analysis
confirmed the persistence of a distinctive viral strain in each of the 2
cases with individual abnormalities within the carboxy terminal region (5
point mutations and a 30-bp deletion for the first case and 6 point
mutations for the second case). The persistence of a given strain in early
and late relapses is evidence towards the view that in Hodgkin's disease
such relapses are related to a single residual tumor cell clone.
Volume 84,
Issue 8,
pp. 2447-2451,
10/15/1994
Copyright © 1994 by The American Society of Hematology
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