|
|
 Previous Article | Table of Contents | Next Article 
Pentoxifylline inhibits integrin-mediated adherence of interleukin-2-
activated human peripheral blood lymphocytes to human umbilical vein
endothelial cells, matrix components, and cultured tumor cells
NL Kovach, CG Lindgren, A Fefer, JA Thompson, T Yednock and JM Harlan
Department of Medicine, University of Washington, Seattle 98195.
Peripheral blood lymphocytes (PBLs) cultured in the presence of recombinant
human interleukin-2 (rhIL-2) develop a natural killer (NK) cell phenotype
(CD16+, CD56+, CD3-) and are referred to as lymphokine- activated killer
cells (LAK). In developing the LAK phenotype, enhanced adherence to matrix
components and endothelial cells have been described. In this report we
investigated the functional behavior of adhesion receptors in
rhIL-2-activated PBLs by in vitro adhesion assay and by flow cytometry.
Compared to PBLs, IL-2-activated PBLs had increased integrin-mediated
adherence to: (1) fibronectin (FN), (2) human umbilical vein endothelial
(HUVE) cells, and (3) cultured melanoma and pancreatic tumor cell lines.
This increase in adherence was mediated by increased surface expression of
members of the beta 1 and beta 2 integrin subfamilies, as determined by
flow cytometric analysis. No induction of an activation-dependent beta 1
(CD29) epitope was detected. We also investigated the effects of the
methylxanthine derivative pentoxifylline (PTX) on PBLs and rhIL-2-activated
PBL adhesion. PBLs co-cultivated in the presence of rhIL-2 (1,000 U/mL) and
PTX exhibited reduced adherence to FN, HUVE and cultured tumor cell lines.
This inhibition by PTX was concentration- and time-dependent. The increased
expression of integrins induced by rhIL-2 was only in part inhibited by
PTX, suggesting that PTX induced a subpopulation of integrins that are
expressed but functionally inactive.
Volume 84,
Issue 7,
pp. 2234-2242,
10/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Dominguez-Jimenez, D. Sancho, M. Nieto, M. C. Montoya, O. Barreiro, F. Sanchez-Madrid, and R. Gonzalez-Amaro
Effect of pentoxifylline on polarization and migration of human leukocytes
J. Leukoc. Biol.,
April 1, 2002;
71(4):
588 - 596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Gonzalez-Amaro, D. Portales-Perez, L. Baranda, J. M. Redondo, S. Martinez-Martinez, M. Yanez-Mo, R. Garcia-Vicuna, C. Cabanas, and F. Sanchez-Madrid
Pentoxifylline Inhibits Adhesion and Activation of Human T Lymphocytes
J. Immunol.,
July 1, 1998;
161(1):
65 - 72.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wang, S. Rath, J. M. Durdik, and R. Sen
Pentoxifylline Inhibits Ig {kappa} Gene Transcription and Rearrangements in Pre-B Cells
J. Immunol.,
February 15, 1998;
160(4):
1789 - 1795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Seki, N. Koyama, N. L. Kovach, T. Yednock, A. W. Clowes, and J. M. Harlan
Regulation of ß1-Integrin Function in Cultured Human Vascular Smooth Muscle Cells
Circ. Res.,
April 1, 1996;
78(4):
596 - 605.
[Abstract]
[Full Text]
|
|
|
|
|
