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Effect of blood flow on thrombin generation is dependent on the nature of
the thrombogenic surface
A Diquelou, S Lemozy, D Dupouy, B Boneu, K Sakariassen and Y Cadroy
Laboratoire d'Hematologie, Hopital Purpan, Toulouse, France.
We have investigated the influence of blood flow on thrombin generation,
fibrin formation, and fibrin deposition on procoagulant and nonprocoagulant
surfaces. Nonanticoagulated human blood was drawn for 5 minutes directly
from an antecubital vein over stimulated endothelial cells expressing
tissue factor and over human type III collagen fibrils, positioned in
parallel-plate perfusion chambers. The shear rates at these surfaces were
50, 650, and 2,600 s-1. Deposition of platelets and fibrin was measured by
morphometry. Thrombin and fibrin formation was determined by measuring
prothrombin fragments 1 + 2 (F 1 + 2), thrombin-antithrombin III complexes,
(T-AT) and fibrinopeptide A (FPA) in blood effluent from the perfusion
chamber at the end of the 5- minute perfusion period. On procoagulant
endothelial cells, the thrombi were primarily composed of fibrin. The
fibrin deposition (81%, 21%, and 2% at 50, 650, and 2,600 s-1,
respectively) and plasma levels of F 1 + 2, T-AT and FPA were shear rate
dependent and highest at 50 s-1. There was a positive correlation between F
1 + 2 and T-AT and the fibrin deposition (P < .01). In contrast, the
collagen surface triggered primarily thrombi that were composed of
platelets. The platelet thrombi and plasma levels of F 1 + 2 and T-AT were
also dependent on the shear rate, but highest at 650 and 2,600 s-1. F 1 + 2
and T-AT reached the same level as observed with procoagulant endothelial
cells at the higher shear rates. There was a positive correlation between F
1 + 2 and T-AT and the platelet thrombus formation (P < .05), confirming
the predominant role of platelets in thrombin generation. Thus, thrombin
formation is strongly influenced by the blood flow, and this effect depends
on the composition of the thrombogenic surface.
Volume 84,
Issue 7,
pp. 2206-2213,
10/01/1994
Copyright © 1994 by The American Society of Hematology
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