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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xie, X Articles by Palacios, R Search for Related Content PubMed PubMed Citation Articles by Xie, X Articles by Palacios, R Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Cloning and expression of a new mammalian chaperonin gene from a multipotent hematopoietic progenitor clone X Xie and R Palacios Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030. Molecular chaperones assist in the folding and assembly of proteins in cells. Although chaperonins have been shown in prokaryotes, mitochondria, and chloroplasts long-ago, a cytoplasmic heteromeric chaperonin complex was isolated only recently and found to contain at least five to six polypeptides, one of which was identified as the product of the T complex polypeptide-1 (TCP-1) gene. We have isolated and cloned a novel gene called A45 from a cDNA library constructed from poly (A)+ RNA of a multipotent hematopoietic progenitor clone. The A45 cDNA encodes a predicted polypeptide of M(r) 58,118 that exhibits 32% overall amino acid sequence identity to TCP-1 and contains the putative adenosine triphosphate-binding domain and two characteristic consensus regions that are conserved in all chaperonins. The A45 gene is expressed in hematopoietic precursors cells at a much higher level than in nonhematopoietic cells and tissues. We conclude that A45 represents a new member of the mammalian chaperonins that is involved in the folding and assembly of polypeptides. Volume 84, Issue 7, pp. 2171-2174, 10/01/1994 Copyright © 1994 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S.-i. Yokota, H. Yanagi, T. Yura, and H. Kubota Cytosolic Chaperonin Is Up-regulated during Cell Growth. PREFERENTIAL EXPRESSION AND BINDING TO TUBULIN AT G1/S TRANSITION THROUGH EARLY S PHASE J. Biol. Chem., December 24, 1999; 274(52): 37070 - 37078. [Abstract] [Full Text] [PDF]

	Copyright © 1994 by American Society of Hematology         Online ISSN: 1528-0020