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A subset of human CD34+ hematopoietic progenitors express low levels of
CD4, the high-affinity receptor for human immunodeficiency virus-type 1
G Zauli, G Furlini, M Vitale, MC Re, D Gibellini, L Zamai, G Visani, P Borgatti, S Capitani and M La Placa
Institute of Human Anatomy, University of Ferrara, Italy.
We investigated the expression of CD4 antigen in normal bone marrow (BM)
samples, enriched in CD34+ hematopoietic progenitor cells. At flow
cytometry, a significant fraction (ranging from 25% to 65%) of CD34+ cells
also showed low levels of CD4 antigen on their surface. The CD4 receptor
densities on the surface of hematopoietic progenitors was approximately 50%
that of peripheral blood monocytes and 5% of peripheral blood T
lymphocytes. In immunoprecipitation experiments, the CD4 antigen expressed
by BM hematopoietic progenitors appeared to be the same form expressed by
mature peripheral blood CD4+ cells and appeared to be a potentially
functional receptor for human immunodeficiency virus-type 1, because it
specifically bound recombinant envelope gp120. Moreover, BM samples, highly
enriched in CD34+ cells, showed the presence of CD4 mRNA at reverse
transcription- polymerase chain reaction examination. In experiments of
complement- mediated cytotoxicity with Leu3-a+Leu3b anti-CD4 monoclonal
antibody, a significant reduction in the number of both classes of
megakaryocyte (burst-forming unit-meg [BFU-meg] and colony-forming unit-meg
[CFU- meg]) and granulocyte/macrophage (CFU-GM) progenitors was observed,
whereas erythroid (BFU-E) progenitors were only slightly affected.
Moreover, purified CD4+ BM cells obtained by immunomagnetic selection,
using high concentrations of Leu3a+Leu3b, showed a colony-forming ability
of megakaryocyte and granulocyte/macrophage progenitors comparable with
that of CD4- BM cells. In conclusion, the present data show that immature
hematopoietic progenitor cells express low levels of CD4, the high-affinity
receptor of human immunodeficiency virus-type 1.
Volume 84,
Issue 6,
pp. 1896-1905,
09/15/1994
Copyright © 1994 by The American Society of Hematology
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