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Transgenic mice carrying the erythropoietin gene promoter linked to lacZ
express the reporter in proximal convoluted tubule cells after hypoxia
F Loya, Y Yang, H Lin, E Goldwasser and M Albitar
Hematopathology Program, University of Texas M.D. Anderson Cancer Center,
Houston 77030.
Results on the localization of erythropoietin (Epo) synthesis in renal
cells have been contradictory, implicating either interstitial or tubular
cells. We fused the lacZ gene to a 7-kb DNA fragment of the mouse Epo gene
encompassing a portion of the first intron, the first exon, and a 6-kb
sequence of the 5'-flanking region. Transgenic mice carrying this construct
show a low level of specific expression of the lacZ gene in proximal
convoluted tubule (PCT) cells. Without hypoxia, no significant expression
was detected in the liver. Hypoxia induced a large degree of lacZ
expression, mainly in kidney PCT cells and to a lesser degree in liver.
However, anemia induced lacZ expression in both kidney and liver. These
findings indicate that, under these conditions, Epo is expressed in tubular
cells, specifically PCT cells.
Volume 84,
Issue 6,
pp. 1831-1836,
09/15/1994
Copyright © 1994 by The American Society of Hematology
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