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Interleukin-4 inhibits the expression of Kit and tryptase during stem cell
factor-dependent development of human mast cells from fetal liver cells
G Nilsson, U Miettinen, T Ishizaka, LK Ashman, AM Irani and LB Schwartz
Department of Medicine, Virginia Commonwealth University, Richmond.
Although interleukin-4 (IL-4) in mice is known to augment the proliferation
of mast cells and to modulate the expression of certain mast cell protease
transcripts, its effect on human mast cells is less well understood. The
current study examined the effects of recombinant human IL-4 (rhuIL-4) on
stem cell factor (SCF)-dependent fetal liver- derived human mast cells in
liquid culture. In no case did rhuIL-4 augment proliferation of mast cells.
rhuIL-4 selectively inhibited certain aspects of the development of mast
cells in cultures of fetal liver cells with rhuSCF. These include lower
numbers and percentages of cells expressing tryptase and surface Kit,
smaller cells, and lower contents of cells for tryptase, histamine, and
Kit. Development of metachromasia was not attenuated. The downregulation of
Kit, the surface receptor for SCF, is probably a critical factor, because
cells lacking this molecule would not be able to respond to SCF. In
contrast to mast cell progenitors, mast cells already developed in vitro
from fetal liver cells are relatively resistant to rhuIL-4, but are still
dependent for survival on the presence of rhuSCF.
Volume 84,
Issue 5,
pp. 1519-1527,
09/01/1994
Copyright © 1994 by The American Society of Hematology
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