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Efficient retrovirus transduction of mouse pluripotent hematopoietic stem
cells mobilized into the peripheral blood by treatment with granulocyte
colony-stimulating factor and stem cell factor
DM Bodine, NE Seidel, MS Gale, AW Nienhuis and D Orlic
Hematopoiesis Section, National Center for Human Genome Research, Bethesda,
MD.
Cytokine-mobilized peripheral blood cells have been shown to participate in
hematopoietic recovery after bone marrow (BM) transplantation, and are
proposed to be useful targets for retrovirus- mediated gene transfer
protocols. We treated mice with granulocyte colony-stimulating factor
(G-CSF) and stem cell factor (SCF) to mobilize hematopoietic progenitor
cells into the peripheral blood. These cells were analyzed for the number
and frequency of pluripotent hematopoietic stem cells (PHSC). We found that
splenectomized animals treated for 5 days with G-CSF and SCF showed a
threefold increase in the absolute number of PHSC over normal mice. The
number of peripheral- blood PHSC increased 250-fold from 29 per untreated
mouse to 7,200 in peripheral-blood PHSC in splenectomized animals treated
for 5 days with G-CSF and SCF. Peripheral blood PHSC mobilized by treatment
with G-CSF and SCF were analyzed for their ability to be transduced by
retroviral vectors. Peripheral-blood PHSC from splenectomized animals G-CSF
and SCF were transduced with a recombinant retrovirus containing the human
MDR-1 gene. The frequency of gene transfer into peripheral blood PHSC from
animals treated for 5 and 7 days was two-fold and threefold higher than
gene transfer into PHSC from the BM of 5-fluorouracil-treated mice (P <
.01). We conclude that peripheral blood stem cells mobilized by treatment
with G-CSF and SCF are excellent targets for retrovirus- mediated gene
transfer.
Volume 84,
Issue 5,
pp. 1482-1491,
09/01/1994
Copyright © 1994 by The American Society of Hematology
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2271 - 2286.
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|
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|
|
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|
|
|
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|
|
|
|
|
|
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|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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1345 - 1364.
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|
|
|
|
|
|
|
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|
|
|
|
|
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March 1, 1997;
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[Abstract]
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|
|
|
|
|
|
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March 1, 1997;
89(5):
1811 - 1817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Uchida, D. He, A. M. Friera, M. Reitsma, D. Sasaki, B. Chen, and A. Tsukamoto
The Unexpected G0/G1 Cell Cycle Status of Mobilized Hematopoietic Stem Cells From Peripheral Blood
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January 15, 1997;
89(2):
465 - 472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Bensinger, R. Clift, C Anasetti, F. Appelbaum, T Demirer, S Rowley, B. Sandmaier, B Torok-Storb, R Storb, and C. Buckner
Transplantation of allogeneic peripheral blood stem cells mobilized by recombinant human granulocyte colony stimulating factor
Stem Cells,
January 1, 1996;
14(1):
90 - 105.
[Abstract]
|
|
|
|
|
|
|
T. Licht, M. Haskins, P. Henthorn, S. E. Kleiman, D. M. Bodine, T. Whitwam, J. M. Puck, M. M. Gottesman, and J. R. Melniczek
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3123 - 3128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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