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Functional differences between CD38- and DR- subfractions of CD34+ bone
marrow cells
LS Rusten, SE Jacobsen, O Kaalhus, OP Veiby, S Funderud and EB Smeland
Department of Immunology, Norwegian Radium Hospital, Oslo.
Several studies have previously demonstrated enrichment in primitive
progenitor cells in subfractions of CD34+ bone marrow (BM) cells not
expressing CD38 or HLA-DR (DR) antigens. However, no studies have directly
compared these two cell populations with regard to their content of
primitive and more committed progenitor cells. Flow cytometric analysis of
immunomagnetic isolated CD34+ cells demonstrated little overlap between
CD34+CD38- and CD34+DR- progenitor subpopulations in that only 12% to 14%
of total CD34+DR- and CD34+CD38- cells were double negative
(CD34+CD38-DR-). Although the number of committed myeloid progenitor cells
(colony-forming units granulocyte- macrophage) was reduced in both
subpopulations, only CD34+CD38- cells were significantly depleted in
committed erythroid progenitor cells (burst-forming units-erythroid). In
single-cell assay, CD34+CD38- cells showed consistently poorer response to
single as opposed to multiple hematopoietic growth factors as compared with
unfractionated CD34+ cells, indicating that the CD34+CD38- subset is
relatively enriched in primitive hematopoietic progenitor cells.
Furthermore, CD34+CD38- and CD34+DR- cells, respectively, formed 3.2-fold
and 1.6-fold more high proliferative potential colony-forming cell
(HPP-CFC) colonies than did unfractionated CD34+ cells. Finally,
CD34+CD38-DR- cells were depleted in HPP-CFCs as compared with
CD34+CD38+DR+ cells. The results of the present study suggest that both the
CD38- and DR- subfractions of CD34+ bone marrow cells are enriched in
primitive hematopoietic progenitor cells, with the CD34+CD38- subpopulation
being more highly enriched than CD34+DR- cells.
Volume 84,
Issue 5,
pp. 1473-1481,
09/01/1994
Copyright © 1994 by The American Society of Hematology
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