|
|
 Previous Article | Table of Contents | Next Article 
Appearance of a megakaryocyte growth-promoting activity, megapoietin,
during acute thrombocytopenia in the rabbit
DJ Kuter and RD Rosenberg
Department of Biology, Massachusetts Institute of Technology, Cambridge
02139.
Plasma obtained from rabbits made thrombocytopenic by the injection of
antiplatelet serum (APS) stimulated megakaryocyte growth in an in vitro
bone marrow culture. Although the number of megakaryocytes that grew was
increased on average to 136%, the major effect was on megakaryocyte ploidy,
which showed a shift in the modal ploidy class from 8N to 16N, an average
change in the geometric mean ploidy from 8.7N to 11.8N, and the appearance
of some 64N megakaryocytes. This in vitro stimulation of megakaryocyte
number and ploidy was shown to be due to the appearance in the circulation
of a positive activity, which we have named megapoietin. Levels of
megapoietin in thrombocytopenic plasma could be quantitated by measuring
the extent of megakaryocyte ploidization in vitro. The relationship over
time of megapoietin to changes in the platelet count was then assessed in a
series of rabbits made thrombocytopenic by APS injection. Although not
elevated after 3 hours of thrombocytopenia, megapoietin increased after 8
hours to 48% of the maximal level, which occurred after 24 hours of
thrombocytopenia. Levels of megapoietin were inversely and proportionally
related to the platelet count during thrombocytopenia and the subsequent
rebound thrombocytosis. During persistent thrombocytopenia, megapoietin
levels remained maximally elevated. These results suggest that megapoietin
may play a physiologic role in the feedback loop between the platelet and
the bone marrow megakaryocytes.
Volume 84,
Issue 5,
pp. 1464-1472,
09/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. T. Kile, A. D. Panopoulos, R. A. Stirzaker, D. F. Hacking, L. H. Tahtamouni, T. A. Willson, L. A. Mielke, K. J. Henley, J.-G. Zhang, I. P. Wicks, et al.
Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia
Blood,
October 1, 2007;
110(7):
2371 - 2380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Feese, T. Tamada, Y. Kato, Y. Maeda, M. Hirose, Y. Matsukura, H. Shigematsu, T. Muto, A. Matsumoto, H. Watarai, et al.
Structure of the receptor-binding domain of human thrombopoietin determined by complexation with a neutralizing antibody fragment
PNAS,
February 17, 2004;
101(7):
1816 - 1821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Kuter and C. G. Begley
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies
Blood,
November 15, 2002;
100(10):
3457 - 3469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Harker, L. K. Roskos, U. M. Marzec, R. A. Carter, J. K. Cherry, B. Sundell, E. N. Cheung, D. Terry, and W. Sheridan
Effects of megakaryocyte growth and development factor on platelet production, platelet life span, and platelet function in healthy human volunteers
Blood,
April 15, 2000;
95(8):
2514 - 2522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Altun, M. Arici, I. C. Haznedaroglu, C. Usalan, Y. Erdem, U. Yasavul, C. Turgan, S. Caglar, and S. Kirazli
Serum thrombopoietin levels in haemodialysis patients: involvement of arteriovenous fistula
Nephrol. Dial. Transplant.,
September 1, 1999;
14(9):
2173 - 2177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Cohen-Solal, N. Vitrat, M. Titeux, W. Vainchenker, and F. Wendling
High-Level Expression of Mpl in Platelets and Megakaryocytes Is Independent of Thrombopoietin
Blood,
May 1, 1999;
93(9):
2859 - 2866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ishida, T. Ito, and S.-i. Kuriya
Effects of C-Mpl Ligand on Cytoplasmic Maturation of Murine Megakaryocytes and on Platelet Production
J. Histochem. Cytochem.,
January 1, 1998;
46(1):
49 - 58.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Horikawa, I. Matsumura, K. Hashimoto, M. Shiraga, S. Kosugi, S. Tadokoro, T. Kato, H. Miyazaki, Y. Tomiyama, Y. Kurata, et al.
Markedly Reduced Expression of Platelet c-mpl Receptor in Essential Thrombocythemia
Blood,
November 15, 1997;
90(10):
4031 - 4038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Shivdasani, P. Fielder, G.-A. Keller, S. H. Orkin, and F. J. de Sauvage
Regulation of the Serum Concentration of Thrombopoietin in Thrombocytopenic NF-E2 Knockout Mice
Blood,
September 1, 1997;
90(5):
1821 - 1827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Kuter
Thrombopoietin: Biology and Clinical Applications
Oncologist,
February 1, 1996;
1(1):
98 - 106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
