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Hypersensitive site 5 of the human beta locus control region functions as a
chromatin insulator
Q Li and G Stamatoyannopoulos
Division of Medical Genetics, University of Washington, Seattle 98195.
The human beta locus control region (LCR) consists of five DNAse I
hypersensitive sites (HS), four of which are erythroid specific and one,
the further upstream located 5'HS5, is constitutive. To characterize the
function of 5'HS5 we analyzed globin gene expression of various constructs
containing HS3 as an enhancer, HS5, and the beta gene as a reporter.
Expression was analyzed in stably transfected MEL cells. We found that the
enhancing effect of hypersensitive site 3 is blocked when the HS5 is
interposed between HS3 and the beta globin gene. These data suggest that
the human 5'HS5 has the properties of a chromatin insulator.
Volume 84,
Issue 5,
pp. 1399-1401,
09/01/1994
Copyright © 1994 by The American Society of Hematology
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