|
|
 Previous Article | Table of Contents | Next Article 
Transcription factor GATA-2 is expressed in erythroid, early myeloid, and
CD34+ human leukemia-derived cell lines
T Nagai, H Harigae, H Ishihara, H Motohashi, N Minegishi, S Tsuchiya, N Hayashi, L Gu, B Andres and JD Engel
Department of Biochemistry, Tohoku University School of Medicine, Sendai,
Japan.
To understand the functional roles that the GATA factors may play during
hematopoietic cell differentiation, we examined the expression of GATA
factor mRNAs and protein products in various human cell lines. Blot
hybridization analyses demonstrated that GATA-1 and GATA-2 mRNAs are
expressed abundantly in a set of cell lines established from human
myelogenous leukemia cells, but the expression pattern of each factor is
distinct. GATA-2 mRNA is expressed in all cell lines tested that express
erythroid markers, and, in addition, the mRNA is also expressed in three
CD34+ cell lines and two early myeloid cell lines. In contrast, the
expression of GATA-1 mRNA showed tight correlation to that of the
erythroid/megakaryocytic lineage markers. We also found that the GATA-2
probe identifies two types of mRNA. Structural analysis of genomic DNA
clones encoding human GATA-2 coupled with RNA blot analysis demonstrated
that there exists an alternative use of polyadenylation consensus sequences
in a single exon and this causes the molecular heterogeneity among GATA-2
mRNAs. Through immunochemical and immunohistochemical analyses using
anti-GATA-1- and anti-GATA-2- specific antibodies, GATA-2 protein was
clearly shown to be present in the nuclei of leukemia-derived early myeloid
and CD34+ cell lines, whereas both GATA-1 and GATA-2 proteins are expressed
in erythroid/megakaryocytic cell lines. Thus, the expression profile of
GATA-2 is consistent with the hypothesis that GATA-2 plays unique roles for
the transcriptional activation of genes in cells at an early stage of
hematopoietic differentiation and in developing cells of the erythroid and
myeloid lineages.
Volume 84,
Issue 4,
pp. 1074-1084,
08/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. Ferreira, A. Wai, R. Shimizu, N. Gillemans, R. Rottier, M. von Lindern, K. Ohneda, F. Grosveld, M. Yamamoto, and S. Philipsen
Dynamic regulation of Gata factor levels is more important than their identity
Blood,
June 15, 2007;
109(12):
5481 - 5490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zheng, K. Kitajima, E. Sakai, T. Kimura, N. Minegishi, M. Yamamoto, and T. Nakano
Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells
Blood,
January 15, 2006;
107(2):
520 - 527.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Oren, I. Torregroza, and T. Evans
An Oct-1 binding site mediates activation of the gata2 promoter by BMP signaling
Nucleic Acids Res.,
August 1, 2005;
33(13):
4357 - 4367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Shimizu, T. Kuroha, O. Ohneda, X. Pan, K. Ohneda, S. Takahashi, S. Philipsen, and M. Yamamoto
Leukemogenesis Caused by Incapacitated GATA-1 Function
Mol. Cell. Biol.,
December 15, 2004;
24(24):
10814 - 10825.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Harigae, O. Nakajima, N. Suwabe, H. Yokoyama, K. Furuyama, T. Sasaki, M. Kaku, M. Yamamoto, and S. Sassa
Aberrant iron accumulation and oxidized status of erythroid-specific delta -aminolevulinate synthase (ALAS2)-deficient definitive erythroblasts
Blood,
February 1, 2003;
101(3):
1188 - 1193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tsuzuki and T. Enver
Interactions of GATA-2 with the promyelocytic leukemia zinc finger (PLZF) protein, its homologue FAZF, and the t(11;17)-generated PLZF-retinoic acid receptor alpha oncoprotein
Blood,
May 1, 2002;
99(9):
3404 - 3410.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Morrison, D. Qian, L. Jerabek, B. A. Thiel, I.-K. Park, P. S. Ford, M. J. Kiel, N. J. Schork, I. L. Weissman, and M. F. Clarke
A Genetic Determinant That Specifically Regulates the Frequency of Hematopoietic Stem Cells
J. Immunol.,
January 15, 2002;
168(2):
635 - 642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi, K. Nishikawa, and M. Yamamoto
Hematopoietic regulatory domain of gata1 gene is positively regulated by GATA1 protein in zebrafish embryos
Development,
June 15, 2001;
128(12):
2341 - 2350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tsuzuki, M. Towatari, H. Saito, and T. Enver
Potentiation of GATA-2 Activity through Interactions with the Promyelocytic Leukemia Protein (PML) and the t(15;17)-Generated PML-Retinoic Acid Receptor alpha Oncoprotein
Mol. Cell. Biol.,
September 1, 2000;
20(17):
6276 - 6286.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Tarumoto, S. Imagawa, K. Ohmine, T. Nagai, M. Higuchi, N. Imai, N. Suzuki, M. Yamamoto, and K. Ozawa
NG-monomethyl-L-arginine inhibits erythropoietin gene expression by stimulating GATA-2
Blood,
September 1, 2000;
96(5):
1716 - 1722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Tang, M. E. Lasbury, D. D. Davidson, M. S. Bartlett, J. W. Smith, and C.-H. Lee
Down-Regulation of GATA-2 Transcription during Pneumocystis carinii Infection
Infect. Immun.,
August 1, 2000;
68(8):
4720 - 4724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takahashi, R. Shimizu, N. Suwabe, T. Kuroha, K. Yoh, J. Ohta, S. Nishimura, K.-C. Lim, J. D. Engel, and M. Yamamoto
GATA factor transgenes under GATA-1 locus control rescue germline GATA-1 mutant deficiencies
Blood,
August 1, 2000;
96(3):
910 - 916.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Moroni, T. Mastrangelo, R. Razzini, L. Cairns, P. Moi, S. Ottolenghi, and B. Giglioni
Regulation of Mouse p45 NF-E2 Transcription by an Erythroid-specific GATA-dependent Intronic Alternative Promoter
J. Biol. Chem.,
March 31, 2000;
275(14):
10567 - 10576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nishimura, S. Takahashi, T. Kuroha, N. Suwabe, T. Nagasawa, C. Trainor, and M. Yamamoto
A GATA Box in the GATA-1 Gene Hematopoietic Enhancer Is a Critical Element in the Network of GATA Factors and Sites That Regulate This Gene
Mol. Cell. Biol.,
January 15, 2000;
20(2):
713 - 723.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Minegishi, J. Ohta, H. Yamagiwa, N. Suzuki, S. Kawauchi, Y. Zhou, S. Takahashi, N. Hayashi, J. D. Engel, and M. Yamamoto
The Mouse GATA-2 Gene is Expressed in the Para-Aortic Splanchnopleura and Aorta-Gonads and Mesonephros Region
Blood,
June 15, 1999;
93(12):
4196 - 4207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Lakshmanan, K. H. Lieuw, K.-C. Lim, Y. Gu, F. Grosveld, J. D. Engel, and A. Karis
Localization of Distant Urogenital System-, Central Nervous System-, and Endocardium-Specific Transcriptional Regulatory Elements in the GATA-3 Locus
Mol. Cell. Biol.,
February 1, 1999;
19(2):
1558 - 1568.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I Pata, M Studer, J. van Doorninck, J Briscoe, S Kuuse, J. Engel, F Grosveld, and A Karis
The transcription factor GATA3 is a downstream effector of Hoxb1 specification in rhombomere 4
Development,
January 12, 1999;
126(23):
5523 - 5531.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Nony, R. Hannon, H. Gould, and G. Felsenfeld
Alternate Promoters and Developmental Modulation of Expression of the Chicken GATA-2 Gene in Hematopoietic Progenitor Cells
J. Biol. Chem.,
December 4, 1998;
273(49):
32910 - 32919.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takahashi, S. Taketani, J.-e. Akasaka, A. Kobayashi, N. Hayashi, M. Yamamoto, and T. Nagai
Differential Regulation of Coproporphyrinogen Oxidase Gene Between Erythroid and Nonerythroid Cells
Blood,
November 1, 1998;
92(9):
3436 - 3444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Minegishi, J. Ohta, N. Suwabe, H. Nakauchi, H. Ishihara, N. Hayashi, and M. Yamamoto
Alternative Promoters Regulate Transcription of the Mouse GATA-2 Gene
J. Biol. Chem.,
February 6, 1998;
273(6):
3625 - 3634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamaguchi, L. I. Zon, S. J. Ackerman, M. Yamamoto, and T. Suda
Forced GATA-1 Expression in the Murine Myeloid Cell Line M1: Induction of c-Mpl Expression and Megakaryocytic/Erythroid Differentiation
Blood,
January 15, 1998;
91(2):
450 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. F. Gordon, S. R. Lewis, B. R. Haugen, R. A. James, M. T. McDermott, W. M. Wood, and E. C. Ridgway
Pit-1 and GATA-2 Interact and Functionally Cooperate to Activate the Thyrotropin beta -Subunit Promoter
J. Biol. Chem.,
September 26, 1997;
272(39):
24339 - 24347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Komatsu, K. Kirito, Y. Kashii, Y. Furukawa, J. Kikuchi, N. Suwabe, M. Yamamoto, and Y. Miura
Cell-Cycle-Dependent Regulation of Erythropoietin Receptor Gene
Blood,
February 15, 1997;
89(4):
1182 - 1188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Perkins and J. H. Kim
Zinc Fingers 1-7 of EVI1 Fail to Bind to the GATA Motif by Itself but Require the Core Site GACAAGATA for Binding
J. Biol. Chem.,
January 12, 1996;
271(2):
1104 - 1110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Towatari, G. E. May, R. Marais, G. R. Perkins, C. J. Marshall, S. Cowley, and T. Enver
Regulation of GATA-2 Phosphorylation by Mitogen-activated Protein Kinase and Interleukin-3
J. Biol. Chem.,
February 24, 1995;
270(8):
4101 - 4107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
