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Coxsackievirus B3 infection in human leukocytes and lymphoid cell lines
T Vuorinen, R Vainionpaa, H Kettinen and T Hyypia
Department of Virology, University of Turku, Finland.
Although coxsackie B viruses (CBVs) are known to cause viremia during acute
infection, the role of the blood cells as a target for virus replication is
poorly understood. We have analyzed the susceptibility of human peripheral
blood mononuclear cells (PBMCs), granulocytes, bone marrow (BM) cells, and
lymphoid cell lines to coxsackievirus B3 infection. Lymphoid cell lines
with B- and T-cell characteristics (Raji and Molt-4, respectively)
supported virus replication to high titers and virus protein synthesis was
detected by metabolic labeling and immunoprecipitation. CBV3 synthesis in
the U937 cell line with mononuclear phagocytic characteristics was very
limited. The virus was able to infect a small proportion of leukocytes and
BM cells, and intracellular virus antigens were detected by
immunofluorescent staining. However, only a diminutive amount of infectious
virus was produced in isolated PBMCs and granulocytes, and no virus protein
synthesis was detected by metabolic labeling and immunoprecipitation in
these cells.
Volume 84,
Issue 3,
pp. 823-829,
08/01/1994
Copyright © 1994 by The American Society of Hematology
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