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Mobilization of long-term hematopoietic reconstituting cells in mice by the
combination of stem cell factor plus granulocyte colony-stimulating factor
XQ Yan, R Briddell, C Hartley, G Stoney, B Samal and I McNiece
Department of Developmental Hematology, Amgen Inc, Thousand Oaks, CA
91320-1789.
In this study, we have compared the ability of recombinant human
granulocyte colony-stimulating factor (rhG-CSF) alone and the combination
of low doses of recombinant rat pegylated stem cell factor (rrSCF-PEG) plus
rhG-CSF to mobilize peripheral blood progenitor cells (PBPCs) with
long-term engrafting potential. Female recipient irradiated mice were
transplanted with PBPCs from male mice that were mobilized with rhG-CSF
alone (group A) or rrSCF-PEG plus rhG-CSF (group B). As previously shown,
greater short-term survival resulted in group B compared with group A, with
80% and 40% survival at 30 days posttransplant, respectively. Both groups
of animals showed long-term donor-derived engraftment in greater than 95%
of animals, as determined by quantitative specific polymerase chain
reaction amplification of a Y chromosome sequence from whole blood of the
mice at 6 to 12 months posttransplantation. Analysis of individual
granulocyte-macrophage colonies, picked up from semisolid methylcellulose
culture of bone marrow cells from transplanted mice, resulted in detection
of donor- derived DNA in 98% of colonies from group B mice compared with
81% from group A mice. These data show that cells with long-term potential
are mobilized by rhG-CSF alone and the combination of rrSCF-PEG plus rhG-
CSF. Furthermore, an increased number of cells with short-term and long-
term engraftment potential was obtained with rrSCF-PEG plus rhG-CSF
compared with rhG-CSF alone.
Volume 84,
Issue 3,
pp. 795-799,
08/01/1994
Copyright © 1994 by The American Society of Hematology
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