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Direct synergistic effects of interleukin-7 on in vitro myelopoiesis of
human CD34+ bone marrow progenitors
FW Jacobsen, LS Rusten and SE Jacobsen
Department of Immunology, Norwegian Radium Hospital, Oslo.
Interleukin-7 (IL-7) is an important growth factor in B and T lymphopoiesis
in mouse and human, whereas IL-7 has been regarded to lack proliferative
effects on cells within the myeloid lineage. However, we have recently
reported that IL-7 potently can enhance colony stimulating factor
(CSF)-induced myelopoiesis from primitive murine hematopoietic progenitors,
showing a novel role of IL-7 in early murine myelopoiesis. Using CD34+
human hematopoietic progenitor cells, we show here a similar role of IL-7
in human myelopoiesis, although interesting differences between the two
species were found as well. Although purified recombinant human (rh)IL-7
alone did not induce any proliferation of CD34+ cells, IL-7 in a
concentration-dependent manner enhanced the colony formation induced by all
four CSFs up to threefold. Furthermore, stem cell factor (SCF)-induced
granulocyte-macrophage (GM) colony formation was increased fourfold in the
presence of IL-7. Single- cell cloning assays showed that these synergistic
effects of IL-7 were directly mediated on the targeted progenitors, and
that IL-7 increased the number, as well as the size of the colonies formed.
Morphological examination showed that IL-7 affected the progeny developed
from CD34+ cells stimulated by G-CSF or IL-3, increasing the number of
CFU-M (colony forming unit-macrophage) and CFU-granulocyte-macrophage,
whereas the number of CFU-granulocyte were unaltered.
Volume 84,
Issue 3,
pp. 775-779,
08/01/1994
Copyright © 1994 by The American Society of Hematology
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