|
|
 Previous Article | Table of Contents | Next Article 
Mac-1 (CD11b/CD18) and CD45 mediate the adhesion of hematopoietic
progenitor cells to stromal cell elements via recognition of stromal
heparan sulfate
DR Coombe, SM Watt and CR Parish
Sir William Dunn School of Pathology, University of Oxford, UK.
Hematopoiesis is regulated by two sets of signals, those generated by
cytokines and those generated when precursor cells interact with bone
marrow (BM) stroma. The intimate contact between precursors and stroma
appears to be mediated by multiple, different receptor-ligand binding
events. To identify receptor-ligand pairs mediating the adhesion of
hematopoietic precursor cells to stroma, an in vitro model of hematopoiesis
was used. This involved coculturing the BM-derived, interleukin-3
(IL-3)-dependent, multipotential cells, FCDP-mix A4 (A4) with a stromal
equivalent embryonic mesenchymal cell line, Swiss 3T3 (3T3). In coculture,
A4 cells survive, proliferate, and differentiate in the absence of
exogenous IL-3, providing they are attached to the 3T3 cell surface. By
using detergent lysates of surface-biotinylated A4 cells, A4 cell molecules
that bind to the stroma could be detected by either fluorescein
isothiocyanate (FITC)-streptavidin or FITC-antibody staining and flow
cytometry. Using this approach the beta 2 integrin, Mac-1, and CD45, a
receptor-type tyrosine phosphatase, were identified as molecules on the A4
cell surface that bind 3T3 cells. Various glycosaminoglycans (GAGs),
particularly heparin and heparan sulfate, blocked binding of A4 cell
surface molecules to the 3T3 cells. The binding of CD45 and Mac-1 to the
3T3 cells was similarly blocked by these GAGs. Removal of heparin-binding
molecules from A4 cell lysates diminished binding to the 3T3 cells and
digestion of the 3T3 cell surface with heparinase abolished the binding of
CD45 and Mac-1. The data suggest that heparan sulfate on the 3T3 cell
surface is a ligand for both CD45 and Mac-1, but the two molecules
recognize different heparan sulfate structural motifs.
Volume 84,
Issue 3,
pp. 739-752,
08/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Vorup-Jensen, L. Chi, L. C. Gjelstrup, U. B. Jensen, C. A. Jewett, C. Xie, M. Shimaoka, R. J. Linhardt, and T. A. Springer
Binding between the Integrin {alpha}Xbeta2 (CD11c/CD18) and Heparin
J. Biol. Chem.,
October 19, 2007;
282(42):
30869 - 30877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Jung, J. Wang, J. Song, Y. Shiozawa, J. Wang, A. Havens, Z. Wang, Y.-X. Sun, S. G. Emerson, P. H. Krebsbach, et al.
Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation
Blood,
July 1, 2007;
110(1):
82 - 90.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Forde, B. J. Tye, S. E. Newey, M. Roubelakis, J. Smythe, C. P. McGuckin, R. Pettengell, and S. M. Watt
Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells
Blood,
March 1, 2007;
109(5):
1825 - 1833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Taichman
Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche
Blood,
April 1, 2005;
105(7):
2631 - 2639.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hidalgo, A. J. Peired, L. A. Weiss, Y. Katayama, and P. S. Frenette
The integrin {alpha}M{beta}2 anchors hematopoietic progenitors in the bone marrow during enforced mobilization
Blood,
August 15, 2004;
104(4):
993 - 1001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Netelenbos, J. van den Born, F. L. Kessler, S. Zweegman, P. C. Huijgens, and A. M. Drager
In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands
J. Leukoc. Biol.,
December 1, 2003;
74(6):
1035 - 1044.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. A. Velders, J. F. M. Pruijt, P. Verzaal, R. van Os, Y. van Kooyk, C. G. Figdor, E.-J. F. M. de Kruijf, R. Willemze, and W. E. Fibbe
Enhancement of G-CSF-induced stem cell mobilization by antibodies against the beta 2 integrins LFA-1 and Mac-1
Blood,
June 17, 2002;
100(1):
327 - 333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Rezzonico, V. Imbert, R. Chicheportiche, and J.-M. Dayer
Ligation of CD11b and CD11c {beta}2 integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1{alpha} (MIP-1{alpha}) and MIP-1{beta} production in primary human monocytes through a pathway dependent on nuclear factor-{kappa}B
Blood,
May 15, 2001;
97(10):
2932 - 2940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Watt, L. H. Butler, M. Tavian, H.-J. Buhring, I. Rappold, P. J. Simmons, A. C. W. Zannettino, D. Buck, A. Fuchs, R. Doyonnas, et al.
Functionally defined CD164 epitopes are expressed on CD34+ cells throughout ontogeny but display distinct distribution patterns in adult hematopoietic and nonhematopoietic tissues
Blood,
May 15, 2000;
95(10):
3113 - 3124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Peter, M. Schwarz, C. Conradt, T. Nordt, M. Moser, W. Kubler, and C. Bode
Heparin Inhibits Ligand Binding to the Leukocyte Integrin Mac-1 (CD11b/CD18)
Circulation,
October 5, 1999;
100(14):
1533 - 1539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamashita, K. Oritani, E. K. Miyoshi, R. Wall, M. Bernfield, and P. W. Kincade
Syndecan-4 Is Expressed by B Lineage Lymphocytes and Can Transmit a Signal for Formation of Dendritic Processes
J. Immunol.,
May 15, 1999;
162(10):
5940 - 5948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Drzeniek, G. Stocker, B. Siebertz, U. Just, T. Schroeder, W. Ostertag, and H.-D. Haubeck
Heparan Sulfate Proteoglycan Expression Is Induced During Early Erythroid Differentiation of Multipotent Hematopoietic Stem Cells
Blood,
May 1, 1999;
93(9):
2884 - 2897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Cortes, F. Deschaseaux, N. Uchida, M.-C. Labastie, A. M. Friera, D. He, P. Charbord, and B. Peault
HCA, an Immunoglobulin-Like Adhesion Molecule Present on the Earliest Human Hematopoietic Precursor Cells, Is Also Expressed by Stromal Cells in Blood-Forming Tissues
Blood,
February 1, 1999;
93(3):
826 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Borghesi, Y. Yamashita, and P. W. Kincade
Heparan Sulfate Proteoglycans Mediate Interleukin-7- Dependent B Lymphopoiesis
Blood,
January 1, 1999;
93(1):
140 - 148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Gupta, T. R. Oegema Jr, J. J. Brazil, A. Z. Dudek, A. Slungaard, and C. M. Verfaillie
Structurally Specific Heparan Sulfates Support Primitive Human Hematopoiesis by Formation of a Multimolecular Stem Cell Niche
Blood,
December 15, 1998;
92(12):
4641 - 4651.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Taichman and S. G. Emerson
The Role of Osteoblasts in the Hematopoietic Microenvironment
Stem Cells,
January 1, 1998;
16(1):
7 - 15.
[Abstract]
[Full Text]
|
|
|
|
|
