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Evidence for nonclonal hematopoietic progenitor cell populations in bone
marrow of patients with myelodysplastic syndromes
H Asano, H Ohashi, M Ichihara, T Kinoshita, T Murate, M Kobayashi, H Saito and T Hotta
First Department of Internal Medicine, Nagoya University School of
Medicine, Japan.
Clonality of marrow hematopoietic progenitor cells in myelodysplastic
syndromes (MDS) was analyzed by X-chromosome inactivation pattern using
polymerase chain reaction (PCR). Five female patients were included in this
study; two with refractory anemia (RA) and three with RA with excess blasts
(RAEB). They were heterozygous for BstXI restriction fragment length
polymorphisms (RFLP) of the X-chromosome-linked phosphoglycerate kinase
(PGK) gene. In each patient, erythroid and nonerythroid colonies, grown in
the presence of erythropoietin and granulocyte-macrophage
colony-stimulating factor (GM-CSF), exhibited no remarkable difference in
clonal constitution. Two patients showed only one methylation pattern,
suggesting the monoclonal origin of hematopoietic progenitor cells.
Colonies of two other patients exhibited predominant and minor methylation
patterns in PGK gene, indicating that nonclonal progenitor cells remain a
minor population. The bone marrow of one patient appeared to contain a
greater proportion of nonclonal progenitors. Stem cell factor (SCF), a
potent colony- stimulating factor, enhanced both erythroid and nonerythroid
colony formation. However, it did not notably alter the clonal
constitutions. We conclude that nonclonal hematopoietic progenitor cells
can persist in a substantial number of MDS patients.
Volume 84,
Issue 2,
pp. 588-594,
07/15/1994
Copyright © 1994 by The American Society of Hematology
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