|
|
 Previous Article | Table of Contents | Next Article 
Fibroblast tissue factor: calcium and ionophore induce shape changes,
release of membrane vesicles, and redistribution of tissue factor antigen
in addition to increased procoagulant activity
SD Carson, GA Perry and SJ Pirruccello
Department of Pathology, University of Nebraska Medical Center, Omaha
68198-6495.
The coagulant activity of tissue factor (TF) in the membranes of cultured
cells is increased after physical disruption of the cells, and a similar
increase can be elicited by treatment of cell cultures with calcium
ionophore and calcium. We observed that the supernatants of cultures
treated with calcium and ionophore 4-Br-A23187 contained more TF activity
than those of control cultures. Phase contrast microscopy showed that
cultures treated with ionophore and calcium contained rounded cells,
membrane vesicles, and cell fragments. Laser-activated fluorescence
microscopy of cells stained for tissue factor antigen showed that
4-Br-A23187, in the presence of 5 mmol/L calcium, caused progressive
changes in cell morphology. Treatment of cultures with thrombin receptor
agonist peptide caused a transient increase in cytoplasmic calcium, but had
no short-term effect on TF activity or cell morphology. These combined
results show that 4-Br-A23187, with extracellular calcium, increases TF
activity concomitant with dramatic changes in cell morphology and the
plasma membrane. The effect of increased cytoplasmic calcium on TF
expression may therefore be similar in mechanism to other models of cell
injury and may be caused by the effects of a sustained increase of
cytosolic calcium on cellular elements that influence membrane stability
and the distribution of TF per se.
Volume 84,
Issue 2,
pp. 526-534,
07/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
U. R. Pendurthi, S. Ghosh, S. K. Mandal, and L. V. M. Rao
Tissue factor activation: is disulfide bond switching a regulatory mechanism?
Blood,
December 1, 2007;
110(12):
3900 - 3908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Fortin, G. E. Rivard, A. Adam, and F. Marceau
Studies on rabbit natural and recombinant tissue factors: intracellular retention and regulation of surface expression in cultured cells
Am J Physiol Heart Circ Physiol,
May 1, 2005;
288(5):
H2192 - H2202.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dorfleutner, E. Hintermann, T. Tarui, Y. Takada, and W. Ruf
Cross-talk of Integrin {alpha}3{beta}1 and Tissue Factor in Cell Migration
Mol. Biol. Cell,
October 1, 2004;
15(10):
4416 - 4425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Aras, A. Shet, R. R. Bach, J. L. Hysjulien, A. Slungaard, R. P. Hebbel, G. Escolar, B. Jilma, and N. S. Key
Induction of microparticle- and cell-associated intravascular tissue factor in human endotoxemia
Blood,
June 15, 2004;
103(12):
4545 - 4553.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Watson, A. K. C. Chan, L. R. Berry, J. Li, S. K. Sood, J. G. Dickhout, L. Xu, G. H. Werstuck, L. Bajzar, H. J. Klamut, et al.
Overexpression of the 78-kDa Glucose-regulated Protein/Immunoglobulin-binding Protein (GRP78/BiP) Inhibits Tissue Factor Procoagulant Activity
J. Biol. Chem.,
May 2, 2003;
278(19):
17438 - 17447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Balasubramanian, E. Grabowski, A. Bini, and Y. Nemerson
Platelets, circulating tissue factor, and fibrin colocalize in ex vivo thrombi: real-time fluorescence images of thrombus formation and propagation under defined flow conditions
Blood,
September 26, 2002;
100(8):
2787 - 2792.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Schecter, B. Spirn, M. Rossikhina, P. L. A. Giesen, V. Bogdanov, J. T. Fallon, E. A. Fisher, L. M. Schnapp, Y. Nemerson, and M. B. Taubman
Release of Active Tissue Factor by Human Arterial Smooth Muscle Cells
Circ. Res.,
July 21, 2000;
87(2):
126 - 132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Petit, P. Lesnik, C. Dachet, M. Moreau, and M. J. Chapman
Tissue Factor Pathway Inhibitor Is Expressed by Human Monocyte–Derived Macrophages : Relationship to Tissue Factor Induction by Cholesterol and Oxidized LDL
Arterioscler. Thromb. Vasc. Biol.,
February 1, 1999;
19(2):
309 - 315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Ott, E. G. Fischer, Y. Miyagi, B. M. Mueller, and W. Ruf
A Role for Tissue Factor in Cell Adhesion and Migration Mediated by Interaction with Actin-binding Protein 280
J. Cell Biol.,
March 9, 1998;
140(5):
1241 - 1253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Bouchard, M. A. Shatos, and P. B. Tracy
Human Brain Pericytes Differentially Regulate Expression of Procoagulant Enzyme Complexes Comprising the Extrinsic Pathway of Blood Coagulation
Arterioscler. Thromb. Vasc. Biol.,
January 1, 1997;
17(1):
1 - 9.
[Abstract]
[Full Text]
|
|
|
|
|
