|
|
 Previous Article | Table of Contents | Next Article 
CD44 can mediate the adhesion of platelets to hyaluronan
I Koshiishi, M Shizari and CB Underhill
Department of Cell Biology, Georgetown University Medical Center,
Washington, DC 20007.
CD44 represents a family of glycoproteins that are present on the surfaces
of some types of lymphocytes, macrophages, and epithelial cells. In the
present study, we have found that CD44 is also present on murine
megakaryocytes and peripheral blood platelets as judged by
immunohistochemical staining. Western blotting of platelet proteins
indicated that this CD44 was predominantly of the 85-kD form. This form of
CD44 also had the capacity to bind hyaluronan, because detergent extracts
of platelets as well as intact platelets could bind soluble [3H]hyaluronan,
and this property was blocked by antibodies directed against CD44. More
importantly, isolated platelets could attach to the hyaluronan-containing
extracellular matrix produced by cultured rat fibrosarcoma cells. This
attachment took place in the absence of divalent cations and could be
blocked by pretreating the rat fibrosarcoma cells with hyaluronidase or by
the addition of an antibody to CD44. These results suggested that CD44 was
responsible for the attachment of platelets to hyaluronan. Histochemical
staining also showed that hyaluronan was present immediately beneath the
endothelial cells of many blood vessels of various tissues, such as the
dermis, lamina propria of the intestinal tract, the lungs, and the
pericardium. Thus, it is possible that CD44 plays an important role in the
attachment of platelets to the surface of exposed connective tissue after
injury to endothelial cells.
Volume 84,
Issue 2,
pp. 390-396,
07/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. R. Taylor and R. L. Gallo
Glycosaminoglycans and their proteoglycans: host-associated molecular patterns for initiation and modulation of inflammation
FASEB J,
January 1, 2006;
20(1):
9 - 22.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. N. Wight and M. J. Merrilees
Proteoglycans in Atherosclerosis and Restenosis: Key Roles for Versican
Circ. Res.,
May 14, 2004;
94(9):
1158 - 1167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. D. Kolodgie, A. P. Burke, A. Farb, D. K. Weber, R. Kutys, T. N. Wight, and R. Virmani
Differential Accumulation of Proteoglycans and Hyaluronan in Culprit Lesions: Insights Into Plaque Erosion
Arterioscler. Thromb. Vasc. Biol.,
October 1, 2002;
22(10):
1642 - 1648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Liu, F. Gao, Z. Han, X. Xu, C. B. Underhill, and L. Zhang
Hyaluronan Synthase 3 Overexpression Promotes the Growth of TSU Prostate Cancer Cells
Cancer Res.,
July 1, 2001;
61(13):
5207 - 5214.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Mummert, M. Mohamadzadeh, D. I. Mummert, N. Mizumoto, and A. Takashima
Development of a Peptide Inhibitor of Hyaluronan-mediated Leukocyte Trafficking
J. Exp. Med.,
September 11, 2000;
192(6):
769 - 780.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Legras, U. Gunthert, R. Stauder, F. Curt, S. Oliferenko, H.C. Kluin-Nelemans, J.P. Marie, S. Proctor, C. Jasmin, and F. Smadja-Joffe
A Strong Expression of CD44-6v Correlates With Shorter Survival of Patients With Acute Myeloid Leukemia
Blood,
May 1, 1998;
91(9):
3401 - 3413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
