|
|
 Previous Article | Table of Contents | Next Article 
Regulation of cytokine expression by interferon-alpha in human bone marrow
stromal cells: inhibition of hematopoietic growth factors and induction of
interleukin-1 receptor antagonist
MJ Aman, U Keller, G Derigs, M Mohamadzadeh, C Huber and C Peschel
Third Department of Medicine, Johannes Gutenberg University School of
Medicine, Mainz, Germany.
We investigated the effects of interferon-alpha (IFN-alpha) on the
expression of cytokines by human bone marrow stromal cells. Production of
granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte-CSF
(G-CSF), and interleukin-1 beta (IL-1 beta) in stromal cell layers was
induced by incubation with IL-1 alpha, tumor necrosis factor (TNF), or
lipopolysaccharide (LPS). Addition of IFN-alpha to such stimulated cultures
resulted in a strong downregulation of mRNA expression of GM-CSF and IL-1
beta. Similarly, the protein levels of GM- CSF and IL-1 beta were
significantly reduced by IFN-alpha, whereas G- CSF production was only
moderately inhibited. In contrast, IFN-alpha markedly stimulated the
production of IL-1 receptor antagonist (IL-1RA) by stromal cells. The
inhibition of cytokine expression resulted in a reduced hematopoietic
activity of stromal cells, indicated by a reduced proliferation of the
factor dependent cell line MO7e on IFN-alpha- treated stromal cells. In the
presence of cycloheximide (CHX), IFN- alpha failed to inhibit IL-1 mRNA
expression, whereas the regulation of GM-CSF and IL-1RA by IFN-alpha was
not affected. Our results indicate that the myelosuppressive effects of
IFN-alpha, as observed in therapeutic applications or associated with viral
infections, are, in part, indirectly mediated by inhibition of the
paracrine production of hematopoietic growth factors.
Volume 84,
Issue 12,
pp. 4142-4150,
12/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Niu, S. Strahotin, B. Hewes, B. Zhang, Y. Zhang, D. Archer, T. Spencer, D. Dillehay, B. Kwon, L. Chen, et al.
Cytokine-Mediated Disruption of Lymphocyte Trafficking, Hemopoiesis, and Induction of Lymphopenia, Anemia, and Thrombocytopenia in Anti-CD137-Treated Mice
J. Immunol.,
April 1, 2007;
178(7):
4194 - 4213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Pedranzini, T. Dechow, M. Berishaj, R. Comenzo, P. Zhou, J. Azare, W. Bornmann, and J. Bromberg
Pyridone 6, A Pan-Janus-Activated Kinase Inhibitor, Induces Growth Inhibition of Multiple Myeloma Cells
Cancer Res.,
October 1, 2006;
66(19):
9714 - 9721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Wang, M. Li, J. J. Rinehart, and R. Zhang
Pretreatment with Dexamethasone Increases Antitumor Activity of Carboplatin and Gemcitabine in Mice Bearing Human Cancer Xenografts: In Vivo Activity, Pharmacokinetics, and Clinical Implications for Cancer Chemotherapy
Clin. Cancer Res.,
March 1, 2004;
10(5):
1633 - 1644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bouchonnet, N. Boechat, M. Bonay, and A. J. Hance
Alpha/Beta Interferon Impairs the Ability of Human Macrophages To Control Growth of Mycobacterium bovis BCG
Infect. Immun.,
June 1, 2002;
70(6):
3020 - 3025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Galon, C. Sudarshan, S. Ito, D. Finbloom, and J. J. O'Shea
IL-12 Induces IFN Regulating Factor-1 (IRF-1) Gene Expression in Human NK and T Cells
J. Immunol.,
June 15, 1999;
162(12):
7256 - 7262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Hermann, M. Rubio, T. Nakajima, G. Delespesse, and M. Sarfati
IFN-{alpha} Priming of Human Monocytes Differentially Regulates Gram-Positive and Gram-Negative Bacteria-Induced IL-10 Release and Selectively Enhances IL-12p70, CD80, and MHC Class I Expression
J. Immunol.,
August 15, 1998;
161(4):
2011 - 2018.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmidt, S. Nagel, J. Proba, C. Thiede, M. Ritter, J. F. Waring, F. Rosenbauer, D. Huhn, B. Wittig, I. Horak, et al.
Lack of Interferon Consensus Sequence Binding Protein (ICSBP) Transcripts in Human Myeloid Leukemias
Blood,
January 1, 1998;
91(1):
22 - 29.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Feldman, T. Ahmed, J.D. Lutton, T. Farley, K. Tani, M. Freund, S. Asano, and N.G. Abraham
Adenovirus Mediated Alpha Interferon (IFN-{alpha}) Gene Transfer into CD34+ Cells and CML Mononuclear Cells
Stem Cells,
September 1, 1997;
15(5):
386 - 395.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Sensebe, M. Deschaseaux, J. Li, P. Herve, and P. Charbord
The Broad Spectrum of Cytokine Gene Expression by Myoid Cells from the Human Marrow Microenvironment
Stem Cells,
March 1, 1997;
15(2):
133 - 143.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. Takai, J. Hara, K. Matsumoto, G. Hosoi, Y. Osugi, A. Tawa, S. Okada, and T. Nakamura
Hepatocyte Growth Factor Is Constitutively Produced by Human Bone Marrow Stromal Cells and Indirectly Promotes Hematopoiesis
Blood,
March 1, 1997;
89(5):
1560 - 1565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Selleri, T. Sato, L. Del Vecchio, L. Luciano, A. J. Barrett, B. Rotoli, N. S. Young, and J. P. Maciejewski
Involvement of Fas-Mediated Apoptosis in the Inhibitory Effects of Interferon-alpha in Chronic Myelogenous Leukemia
Blood,
February 1, 1997;
89(3):
957 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
