|
|
 Previous Article | Table of Contents | Next Article 
Regulation of interleukin-11 protein and mRNA expression in neonatal and
adult fibroblasts and endothelial cells
Y Suen, M Chang, SM Lee, JS Buzby and MS Cairo
Division of Hematology/Oncology and Bone Marrow Transplantation, Children's
Hospital of Orange County, CA 92668.
Interleukin-11 (IL-11), a newly-identified cytokine produced by stromal
cells, elevates platelet counts in neonatal rats in vivo and synergizes in
vitro with IL-3 in supporting murine megakaryocyte colony formation and
stimulating hematopoietic stem cells. Megakaryocytopoiesis is also enhanced
by other colony-stimulating factors (CSFs), including IL-3, IL- 6, and
Steel factor (SLF). Dysregulation of neonatal thrombopoiesis predisposes
newborns to develop thrombocytopenia during sepsis, despite increased
circulating pools of committed thrombopoietic progenitors in newborn cord
blood compared with adult. We previously reported reduced expression of
granulocyte-macrophage colony-stimulating factor (GM- CSF),
granulocyte-colony-stimulating factor (G-CSF), and IL-3 from stimulated
cord mononuclear cells, but increased expression of SLF in human umbilical
vein endothelial cells (HUVEC). Therefore, we hypothesized that IL-3, IL-6,
and SLF might modulate megakaryocytopoiesis by inducing IL-11 expression,
and newborns might express altered levels of IL-11 mRNA expression during
activated conditions, contributing to the difference in circulating colony-
forming unit-megakaryocyte (CFU-Meg) cord and adult blood. Phorbol
myristate acetate (PMA) induced a twofold greater increase in IL-11 mRNA
expression in neonatal fibroblasts (NFb) compared with adult fibroblasts
(AFb), and a 3.6-fold greater increase in HUVEC than human adult aorta
endothelial cells (HAEC) by Northern blot analysis. PMA also induced a
threefold greater increase in IL-11 protein production in NFb than AFb.
Physiologic agonists IL-1 alpha, transforming growth factor-beta 1
(TGF-beta 1), and TGF-beta 2 triggered upregulation of IL- 11 mRNA
expression in both NFb and AFb. However, IL-3, IL-6, PIXY321 (a GM-CSF-IL-3
fusion protein), and SLF failed to upregulate IL-11 mRNA expression from
the basal level, while macrophage-colony stimulating factor (M-CSF) mRNA
was significantly induced. These data suggest that the hematopoietic effect
of IL-6, SLF, and IL-3 on megakaryocytopoiesis is probably not mediated by
secondary IL-11 mRNA expression. Similarly, inflammatory agonists IL-1
beta, lipopolysaccharide (LPS), and tumor necrosis factor-alpha (TNF-alpha)
alone did not upregulate IL-11 expression from the basal level in
endothelial cells, whereas intracellular adhesion molecule-1 (ICAM-1) and
endothelial leukocyte adhesion molecule-1 were strongly induced. Minimal
basal IL-11 expression was detected by reverse transcriptase-polymerase
chain reaction (RT-PCR) in NFb, AFb, HUVEC and HAEC. The quantitative
RT-PCR assay also verified that IL-1 beta and TNF-alpha-stimulated HUVEC
and HAEC, and IL-3- and IL-6-stimulated NFb and AFb only expressed minimal
levels of IL-11 mRNA.(ABSTRACT TRUNCATED AT 400 WORDS)
Volume 84,
Issue 12,
pp. 4125-4134,
12/15/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Jiang, C. Van de Ven, P. Satwani, L. V. Baxi, and M. S. Cairo
Differential Gene Expression Patterns by Oligonucleotide Microarray of Basal versus Lipopolysaccharide-Activated Monocytes from Cord Blood versus Adult Peripheral Blood
J. Immunol.,
May 15, 2004;
172(10):
5870 - 5879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bamba, A. Andoh, H. Yasui, J. Makino, S. Kim, and Y. Fujiyama
Regulation of IL-11 expression in intestinal myofibroblasts: role of c-Jun AP-1- and MAPK-dependent pathways
Am J Physiol Gastrointest Liver Physiol,
August 8, 2003;
285(3):
G529 - G538.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chen, P. R. Sanberg, Y. Li, L. Wang, M. Lu, A. E. Willing, J. Sanchez-Ramos, and M. Chopp
Intravenous Administration of Human Umbilical Cord Blood Reduces Behavioral Deficits After Stroke in Rats
Stroke,
November 1, 2001;
32(11):
2682 - 2688.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E M Nilsen, F-E Johansen, F L Jahnsen, K E A Lundin, T Scholz, P Brandtzaeg, and G Haraldsen
Cytokine profiles of cultured microvascular endothelial cells from the human intestine
Gut,
May 1, 1998;
42(5):
635 - 642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Cairo and J. E. Wagner
Placental and/or Umbilical Cord Blood: An Alternative Source of Hematopoietic Stem Cells for Transplantation
Blood,
December 15, 1997;
90(12):
4665 - 4678.
[Full Text]
[PDF]
|
|
|
|
|
