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Synergistic actions of stem cell factor and other burst-promoting
activities on proliferation of CD34+ highly purified blood progenitors
expressing HLA-DR or different levels of c-kit protein
Y Sonoda, H Sakabe, Y Ohmisono, S Tanimukai, S Yokota, S Nakagawa, SC Clark and T Abe
Department of Hygiene, Kyoto Prefectural University of Medicine, Japan.
We studied the synergistic effects of stem cell factor (SCF) and other
burst-promoting activities (BPAs) such as interleukin-3 (IL-3),
granulocyte-macrophage colony-stimulating factor (GM-CSF), or IL-9 on
proliferation of human peripheral blood-derived highly purified
progenitors. SCF, IL-3, GM-CSF, and IL-9 showed significant BPA when
CD34+HLA-DR+ cells were used as the target population. IL-3 exerted the
most potent BPA, and GM-CSF supported approximately 40% to 70% of the
erythroid burst-forming units that are responsive to IL-3. SCF and IL-9
showed much weaker BPA than that of IL-3 or GM-CSF. Combinations of IL- 3
with other BPAs did not show synergistic actions supporting erythroid-
burst formation. However, GM-CSF showed a significant additive effect with
IL-9 or SCF. When CD34+c-kithigh cells were used as the target, SCF showed
a much stronger BPA. Also, a distinct additive effect between SCF and IL-3
or GM-CSF on erythrocyte-containing mixed colony formation was observed. On
the other hand, when CD34+c-kitlow cells were used as the target, SCF,
IL-3, and GM-CSF could express BPA. In contrast, IL-9 alone failed to
support erythroid-burst formation. Because CD34+c-kithigh cells weakly
expressed CD34 antigen, these cells appeared to be more mature progenitors
than CD34+c-kitlow cells. These results suggest that IL-9 acts on more
mature progenitors than those of SCF, IL-3, or GM-CSF and that the primary
target of SCF is multipotential progenitors at the very early stage of
development.
Volume 84,
Issue 12,
pp. 4099-4106,
12/15/1994
Copyright © 1994 by The American Society of Hematology
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