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Monoclonal antibodies directed against the Epstein-Barr virus-encoded
nuclear antigen 1 (EBNA1): immunohistologic detection of EBNA1 in the
malignant cells of Hodgkin's disease
FA Grasser, PG Murray, E Kremmer, K Klein, K Remberger, W Feiden, G Reynolds, G Niedobitek, LS Young and N Mueller-Lantzsch
Abteilung Virologie, Universitatskliniken des Saarlandes, Homburg, Germany.
Monoclonal antibodies directed against the Epstein-Barr virus nuclear
protein 1 (EBNA1) were used to examine conventional paraffin sections from
a series of EBV-associated lymphoproliferative disorders by
immunohistochemistry. The presence of latent EBV infection in tumor cells
was determined by in situ hybridization for the Epstein-Barr virus early
RNAs (EBERs). Of those EBER-positive cases a total of 28 of 40 cases of
Hodgkin's disease, 3 of 3 cases of Burkitt's lymphoma, and 8 of 8 cases of
human immunodeficiency virus-associated cerebral B-cell lymphoma expressed
detectable amounts of EBNA1. In the positive cases, expression was confined
to the tumor cells. No reactivity was detected in EBV-negative cases of the
above tumors or in 8 cases of EBV-negative cases of large cell anaplastic
non-Hodgkin lymphoma. This report provides the first unequivocal evidence
for the expression of the EBNA1 protein in the tumor cells of Hodgkin's
disease and validates an important reagent with which to analyze the role
of EBV in various virus-associated malignancies.
Volume 84,
Issue 11,
pp. 3792-3798,
12/01/1994
Copyright © 1994 by The American Society of Hematology
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