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Diagnosis of t(2;5)(p23;q35)-associated Ki-1 lymphoma with
immunohistochemistry
M Shiota, J Fujimoto, M Takenaga, H Satoh, R Ichinohasama, M Abe, M Nakano, T Yamamoto and S Mori
Department of Pathology and Oncology, Institute of Medical Science,
University of Tokyo, Japan.
Some Ki-1 lymphomas carry a specific chromosomal translocation,
t(2;5)(p23;q35). We have recently found a novel hyperphosphorylated 80- kD
protein tyrosine kinase, p80, in a human Ki-1 lymphoma with this
translocation. Subsequent cDNA cloning showed that p80 is a fusion protein
of two different genes on chromosome 2p23 and 5q35, the novel tyrosine
kinase gene and nucleophosmin gene, respectively. In this study, we
intended to detect p80 on lymphoma tissues with immunologic methods. Thus,
we developed rabbit polyclonal antibody using a synthetic peptide
corresponding to a part of its kinase domain. The antibody (anti-p80)
immunoprecipitated and immunoblotted p80 specifically from AMS3. Then, to
examine whether t(2;5)(p23;q35) was present on biopsied lymphomas, reverse
transcriptase-polymerase chain reaction (RT-PCR) covering the fusion
junction of p80 mRNA was performed. Among 10 Ki-1 lymphomas and 10
additional lymphomas other than the Ki-1 lymphomas, expression of p80 mRNA
was detected in three cases exclusively. When these 20 cases and additional
30 lymphomas were immunostained with anti-p80, positive staining was noted
exclusively in the three cases found by PCR to have harbored the p80 mRNA.
Thus, the present immunostaining, as well as PCR, was shown to be efficient
for detecting lymphomas producing this chimeric protein/mRNA.
Volume 84,
Issue 11,
pp. 3648-3652,
12/01/1994
Copyright © 1994 by The American Society of Hematology
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