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Mast cell growth factor modulates CD36 antigen expression on erythroid
progenitors from human bone marrow and peripheral blood associated with
ongoing differentiation
JT de Wolf, EW Muller, DH Hendriks, RM Halie and E Vellenga
Department of Hematology, University Hospital Groningen, The Netherlands.
To study the differentiation process of erythroid progenitors from normal
human bone marrow and peripheral blood, CD34/CD36 sorted cells were
cultured in the presence of Erythropoietin (Epo) and Epo plus mast cell
growth factor (MGF). The CD34+/CD36- cell fraction from bone marrow
supported 74 +/- 33 erythroid burst forming units (BFU-E)/10(4) cells (mean
+/- SD, n = 4) in the presence of Epo, which increased 2.1- fold by
coculturing with MGF. However, erythroid colony-forming units (CFU-E) were
not cultured from the CD34+/CD36- cell fraction. In contrast, the
CD34-/CD36+ cell fraction supported CFU-Es in the presence of Epo (152 +/-
115/10(5)) or Epo plus MGF (180 +/- 112/10(5)), whereas BFU-Es were hardly
noticed. However, the transition of the BFu-E to CFU-E was observed by
incubating CD34+/CD36- cells (10(4)/100 microL) in suspension with Epo plus
MGF for 7 days followed by Epo in the colony assay. This was reflected by
the appearance of CD34-/CD36+/Glycophorin A+/CD14- cells. In addition high
numbers of CFU- Es (1,000 +/- 150, n = 4) were cultured from this cell
fraction. In contrast to bone marrow erythroid progenitors, no peripheral
blood CFU- Es were cultured from either the CD36+ or CD36- fraction,
whereas BFU- Es were predominantly present in the CD36+ fraction. However,
the CD34+ progenitor cell from peripheral blood did have intrinsic capacity
to differentiate to CFU-Es because CD34+/CD36- cells incubated with Epo
plus MGF for 7 days and followed by Epo in the colony assay, supported high
numbers of CFU-Es (1,200 +/- 400, n = 3). To study whether additional
growth factors have similar effects on erythroid progenitors, experiments
were performed with interleukin 1 (IL-1), IL- 3, and IL-6. IL-1 and IL-6
did not modulate the Epo supported proliferation and differentiation. In
contrast, IL-3 in the presence of Epo did support CFU-Es, from CD34+/CD36-
cells after 7 days in suspension culture. However, flow cytometry analysis
showed that Epo plus IL-3 not only supported CD34-/CD36+/Glycophorin A+
cells but also CD36+/CD14+ cells, indicating the differentiation along
different cell lineages. In summary, the data show a phenotypic distinction
between bone marrow and peripheral blood erythroid progenitors with regard
to CD36 expression. In addition, the results suggest that Epo plus MGF or
IL-3 and preincubation in suspension culture are prerequisites for the
transition of the BFU-E to the CFU-E.
Volume 84,
Issue 1,
pp. 59-64,
07/01/1994
Copyright © 1994 by The American Society of Hematology
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