|
|
 Previous Article | Table of Contents | Next Article 
The N-domain of the biliary glycoprotein (BGP) adhesion molecule mediates
homotypic binding: domain interactions and epitope analysis of BGPc
AM Teixeira, J Fawcett, DL Simmons and SM Watt
ICRF Medical Oncology Unit, St Bartholomew's Hospital, London, UK.
The biliary glycoproteins (BGPs) represent a group of at least eight
differentially spliced molecules belonging to the carcinoembryonic antigen
(CEA) subgroup of the CEA family. These molecules are recognized by the
CD66 monoclonal antibodies (MoAbs) and function as homotypic and
heterotypic adhesion molecules. The extracellular region of the BGPc splice
variant comprises an N-terminal IgV-like domain and three IgC2-set domains
(A1, B1, and A2). Using soluble recombinant BGP domain variants, we
demonstrate in this report that the N-terminal domain mediates homotypic
adhesion. Furthermore, this adhesion is both temperature- and
cation-dependent. The soluble domain variants of BGP are ideal molecules
for epitope mapping. Using these constructs, we have mapped 11 MoAbs that
react with the CEA family to different domains of BGPc and have shown that
the CD66 MoAbs, YTH71.3.2 and CLBgran 10 (M38), recognize epitopes in the
N-terminal domain.
Volume 84,
Issue 1,
pp. 211-219,
07/01/1994
Copyright © 1994 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. L. Servin
Pathogenesis of Afa/Dr Diffusely Adhering Escherichia coli
Clin. Microbiol. Rev.,
April 1, 2005;
18(2):
264 - 292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Comegys, S.-H. Lin, D. Rand, D. Britt, D. Flanagan, H. Callanan, K. Brilliant, and D. C. Hixson
Two Variable Regions in Carcinoembryonic Antigen-related Cell Adhesion Molecule1 N-terminal Domains Located in or Next to Monoclonal Antibody and Adhesion Epitopes Show Evidence of Recombination in Rat but Not in Human
J. Biol. Chem.,
August 13, 2004;
279(33):
35063 - 35078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kirshner, C.-J. Chen, P. Liu, J. Huang, and J. E. Shively
CEACAM1-4S, a cell-cell adhesion molecule, mediates apoptosis and reverts mammary carcinoma cells to a normal morphogenic phenotype in a 3D culture
PNAS,
January 21, 2003;
100(2):
521 - 526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Markel, N. Lieberman, G. Katz, T. I. Arnon, M. Lotem, O. Drize, R. S. Blumberg, E. Bar-Haim, R. Mader, L. Eisenbach, et al.
CD66a Interactions Between Human Melanoma and NK Cells: A Novel Class I MHC-Independent Inhibitory Mechanism of Cytotoxicity
J. Immunol.,
March 15, 2002;
168(6):
2803 - 2810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nakajima, H. Iijima, M. F. Neurath, T. Nagaishi, E. E. S. Nieuwenhuis, R. Raychowdhury, J. Glickman, D. M. Blau, S. Russell, K. V. Holmes, et al.
Activation-Induced Expression of Carcinoembryonic Antigen-Cell Adhesion Molecule 1 Regulates Mouse T Lymphocyte Function
J. Immunol.,
February 1, 2002;
168(3):
1028 - 1035.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kuroki, H. Abe, T. Imakiirei, S. Liao, H. Uchida, Y. Yamauchi, S. Oikawa, and M. Kuroki
Identification and comparison of residues critical for cell-adhesion activities of two neutrophil CD66 antigens, CEACAM6 and CEACAM8
J. Leukoc. Biol.,
October 1, 2001;
70(4):
543 - 550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Watt, A. M. Teixeira, G.-Q. Zhou, R. Doyonnas, Y. Zhang, F. Grunert, R. S. Blumberg, M. Kuroki, K. M. Skubitz, and P. A. Bates
Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site
Blood,
September 1, 2001;
98(5):
1469 - 1479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Doyonnas, J. Yi-Hsin Chan, L. H. Butler, I. Rappold, J. E. Lee-Prudhoe, A. C. W. Zannettino, P. J. Simmons, H.-J. Buhring, J.-P. Levesque, and S. M. Watt
CD164 Monoclonal Antibodies That Block Hemopoietic Progenitor Cell Adhesion and Proliferation Interact with the First Mucin Domain of the CD164 Receptor
J. Immunol.,
July 15, 2000;
165(2):
840 - 851.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. Skubitz, K. D. Campbell, and A. P. N. Skubitz
Synthetic Peptides of CD66a Stimulate Neutrophil Adhesion to Endothelial Cells
J. Immunol.,
April 15, 2000;
164(8):
4257 - 4264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. M. Morales, A. Christ, S. M. Watt, H. S. Kim, K. W. Johnson, N. Utku, A. M. Texieira, A. Mizoguchi, E. Mizoguchi, G. J. Russell, et al.
Regulation of Human Intestinal Intraepithelial Lymphocyte Cytolytic Function by Biliary Glycoprotein (CD66a)
J. Immunol.,
August 1, 1999;
163(3):
1363 - 1370.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Quill and D. L. Garbers
Sperad Is a Novel Sperm-specific Plasma Membrane Protein Homologous to a Family of Cell Adhesion Proteins
J. Biol. Chem.,
December 27, 1996;
271(52):
33509 - 33514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
